Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
FEBS Lett. 2013 Jul 11;587(14):2247-53. doi: 10.1016/j.febslet.2013.05.054. Epub 2013 Jun 4.
Our study demonstrated the functions of microRNA-7 (miR-7) in cervical cancer. The overexpression of miR-7 in the cervical cancer cell lines HeLa and C-33A suppressed cell viability and promoted cell apoptosis, whereas the inhibition of miR-7 had opposite effects. Furthermore, an oncogene, X-linked inhibitor of apoptosis protein (XIAP), was identified as a new target of miR-7, and the ectopic expression of XIAP rescued the effects induced by miR-7 in HeLa and C-33A cells. These results indicate that miR-7 targeted and downregulated the oncogene XIAP to regulate the effect of miR-7 on apoptosis and malignant behaviors of HeLa and C-33A cells.
我们的研究证明了 microRNA-7（miR-7）在宫颈癌中的功能。在宫颈癌细胞系 HeLa 和 C-33A 中过表达 miR-7 抑制细胞活力并促进细胞凋亡，而 miR-7 的抑制则产生相反的效果。此外，一种癌基因 X 连锁凋亡抑制蛋白（XIAP）被鉴定为 miR-7 的新靶标，XIAP 的异位表达挽救了 miR-7 在 HeLa 和 C-33A 细胞中诱导的效应。这些结果表明，miR-7 靶向并下调癌基因 XIAP 以调节 miR-7 对 HeLa 和 C-33A 细胞凋亡和恶性行为的影响。
