The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Hong Kong Special Administrative Region.
Mar Pollut Bull. 2013 Aug 15;73(1):273-81. doi: 10.1016/j.marpolbul.2013.05.009. Epub 2013 Jun 3.
Man-made polybrominated diphenyl ethers (PBDEs) used as flame retardants in various consumer products may be harmful to marine organisms. Larvae of some marine invertebrates, especially invasive species, can develop resistance to PBDEs through altered protein expression patterns or proteome plasticity. This is the first report of a proteomics approach to study BDE-47 induced molecular changes in the invasive limpet Crepidula onyx. Larvae of C. onyx were cultured for 5 days (hatching to metamorphosis) in the presence of BDE-47 (1 μg L(-1)). Using a 2-DE proteomics approach with triple quadrupole and high-resolution TOF-MS, we showed that BDE-47 altered the proteome structure but not the growth or metamorphosis of C. onyx larvae. We found eight significant differentially expressed proteins in response to BDE-47, deemed the protein expression signature, consisting of cytoskeletal, stress tolerance, metabolism and energy production related proteins. Our data suggest C. onyx larvae have adequate proteome plasticity to tolerate BDE-47 toxicity.
人工合成的多溴二苯醚（PBDEs）作为各种消费品中的阻燃剂可能对海洋生物有害。一些海洋无脊椎动物的幼虫，特别是入侵物种，可以通过改变蛋白质表达模式或蛋白质组可塑性对 PBDEs 产生抗性。这是首次采用蛋白质组学方法研究 BDE-47 对入侵石鳖 Crepidula onyx 分子变化的影响。在存在 BDE-47（1 μg/L）的情况下，将 C.onyx 的幼虫培养 5 天（孵化到变态）。使用三重四极杆和高分辨率 TOF-MS 的 2-DE 蛋白质组学方法，我们表明 BDE-47 改变了蛋白质组结构，但不影响 C.onyx 幼虫的生长或变态。我们发现了 8 种对 BDE-47 有显著差异表达的蛋白质，认为是蛋白质表达特征，包括细胞骨架、应激耐受、代谢和能量产生相关的蛋白质。我们的数据表明，C.onyx 幼虫有足够的蛋白质组可塑性来耐受 BDE-47 毒性。
