Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China,
Environ Sci Pollut Res Int. 2013 Dec;20(12):8831-8. doi: 10.1007/s11356-013-1852-1. Epub 2013 Jun 7.
ZJ0273 (propyl 4-(2-(4,6-demethoxy pyrimidin-2-yloxy)benzylamino)benzoate) is a novel herbicide developed in China for oilseed crop. Sixteen bacteria capable of utilizing ZJ0273 as the sole carbon source were isolated from soils. One of the isolates was designated as Bacillus sp. CY based on its physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA sequences. The present study aimed to investigate the ZJ0273 degradation characteristics and kinetics by Bacillus sp. CY which has the ability to utilize ZJ0273 as the sole source of carbon and energy under aerobic conditions. The optimum biodegradation temperature, pH, and ZJ0273 initial concentration were 20-40 °C, 5.0-9.0, and 50-400 mg/l, respectively. Strain CY degraded 65 % of ZJ0273 (initial concentration of 50 mg/l) during 30 days of incubation in basal mineral medium at pH 8.0 and 35 °C. DT50 (half-life value), k (degradation rate constant of ZJ0273), and R (2) are 19.20 days, 0.0361 day(-1), and 0.9464, respectively.
ZJ0273（丙基 4-（2-（4,6-二甲氧基嘧啶-2-基氧基）苄基氨基）苯甲酸酯）是中国开发的一种新型除草剂，用于油料作物。从土壤中分离到了 16 种能够利用 ZJ0273 作为唯一碳源的细菌。其中一个分离株被命名为芽孢杆菌 sp. CY，基于其生理生化特性和 16S rDNA 序列的系统发育分析。本研究旨在研究枯草芽孢杆菌 CY 利用 ZJ0273 作为唯一碳源和能源在有氧条件下的 ZJ0273 降解特性和动力学。最佳生物降解温度、pH 值和 ZJ0273 初始浓度分别为 20-40°C、5.0-9.0 和 50-400mg/l。在 pH 8.0 和 35°C 的基础矿物培养基中孵育 30 天，菌株 CY 降解了初始浓度为 50mg/l 的 ZJ0273 的 65%。DT50（半衰期值）、k（ZJ0273 的降解速率常数）和 R（2）分别为 19.20 天、0.0361 天（-1）和 0.9464。
