Fei Qianjin, Jin Jianyuan, Ni Wuhua, Huang Xuefen
Department of Reproductive Medicine, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325000, P.R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2013 Jun;30(3):357-61. doi: 10.3760/cma.j.issn.1003-9406.2013.03.023.
To investigate variation of sperm DNA fragmentation index (DFI) in male partners of infertile couples.
A total of 539 males between April 2009 and April 2012 were analyzed. At least one repeated routine semen analysis and sperm DNA fragmentation test were performed for each sample by sperm chromatin dispersion (SCD) analysis following World Health Organization guidelines. Coefficient of variation (CV) for DFI was calculated.
Respectively, 1, 2, 3 and 4 repeated SCD analyses were carried out on 473, 59, 6 and 1 semen samples. The median interval between the first and repeated SCD measurements was 3.0 (1.0-11.0) months. For the first tested samples, the between-sample coefficient of variation (CVB) for DFI was 71.2%. A significant difference has been found between DFI of the first measurement and DFI of repeated measurement in 0.5 to 3 months, 3 to 12 months and 12 to 34 months (P< 0.01). Compared with the first test, 26.3% of males were on both sides of the cut-off point of 18%. The median within-subject coefficient of variation (CVw) for DFI of 539 men was 26.0% (12.6%-42.8%). And the median CVw DFI was significantly lower compared with CVw of sperm count, concentration, progressive motility and normal morphology (P< 0.01). Significant correlations were found between the CVw DFI and sperm count, concentration and interval among the samples (P< 0.05).
DFI of male partners for infertile couples is a parameter with substantial variation, repeated SCD measurements are therefore recommended.
研究不育夫妇男性伴侣精子DNA碎片化指数（DFI）的变化情况。
对2009年4月至2012年4月期间的539名男性进行分析。按照世界卫生组织指南，对每个样本至少进行一次重复的常规精液分析和精子DNA碎片化检测，采用精子染色质扩散（SCD）分析法。计算DFI的变异系数（CV）。
分别对473、59、6和1份精液样本进行了1、2、3和4次重复的SCD分析。首次和重复SCD测量之间的中位间隔时间为3.0（1.0 - 11.0）个月。对于首次检测的样本，DFI的样本间变异系数（CVB）为71.2%。在0.5至3个月、3至12个月和12至34个月时，首次测量的DFI与重复测量的DFI之间存在显著差异（P < 0.01）。与首次检测相比，26.3%的男性处于18%的临界值两侧。539名男性DFI的受试者内变异系数（CVw）中位数为26.0%（12.6% - 42.8%）。并且DFI的CVw中位数显著低于精子计数、浓度、前向运动率和正常形态的CVw（P < 0.01）。DFI的CVw与精子计数、浓度以及样本间间隔存在显著相关性（P < 0.05）。
不育夫妇男性伴侣的DFI是一个具有显著变异的参数，因此建议进行重复的SCD测量。
