白杨素通过 ERK/MAPK 激活促进成骨分化。
Chrysin promotes osteogenic differentiation via ERK/MAPK activation.
机构信息
Protein & Peptide Pharmaceutical Laboratory, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
出版信息
Protein Cell. 2013 Jul;4(7):539-47. doi: 10.1007/s13238-013-3003-3. Epub 2013 Jun 6.
Abstract
The effect of the anti-inflammatory flavonoid chrysin on osteogenesis was determined in preosteoblast MC3T3-E1 cells. Results demonstrated that chrysin could induce osteogenic differentiation in the absence of other osteogenic agents. Chrysin treatment promoted the expression of transcription factors (Runx2 and Osx) and bone formation marker genes (Col1A1, OCN, and OPN) as well as enhanced the formation of mineralized nodules. During osteogenic differentiation, chrysin preferentially activated ERK1/2, but not JNK nor the p38 MAPKs. Further experiments with inhibitors revealed the co-treatment of U0126, PD98059, or ICI182780 (a general ER antagonist) with chrysin effectively abrogated the chrysin-induced osteogenesis and ERK1/2 activation. Thus, the effect of chrysin on osteogenesis is ERK1/2-dependent and involves ER. Therefore, chrysin has the significant potential to enhance osteogenesis for osteoporosis prevention and treatment.
摘要
研究了具有抗炎作用的黄酮类化合物白杨素对成骨的影响。结果表明，白杨素在没有其他成骨剂的情况下可以诱导成骨分化。白杨素处理促进了转录因子（Runx2 和 Osx）和骨形成标志物基因（Col1A1、OCN 和 OPN）的表达，并增强了矿化结节的形成。在成骨分化过程中，白杨素优先激活 ERK1/2，但不激活 JNK 或 p38MAPKs。用抑制剂进行的进一步实验表明，用 U0126、PD98059 或 ICI182780（一种通用的 ER 拮抗剂）与白杨素共同处理可有效阻断白杨素诱导的成骨作用和 ERK1/2 激活。因此，白杨素对成骨的作用是 ERK1/2 依赖性的，并涉及 ER。因此，白杨素具有增强成骨作用的潜力，可用于预防和治疗骨质疏松症。
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