Mohedano M, Guzman R, Arrabal R, López Lacomba J-L, Matykina E
Departamento de Ciencia de Materiales, Facultad de Ciencias Químicas, Universidad Complutense, 28040, Madrid, Spain.
J Biomed Mater Res B Appl Biomater. 2013 Nov;101(8):1524-37. doi: 10.1002/jbm.b.32974. Epub 2013 Jun 7.
A Plasma electrolytic oxidation (PEO) process was used to produce bioactive coatings on Ti. PEO coatings with Ca/P atomic ratio of 1.7 and 4.0 were fabricated and characterized with respect to their morphology, composition, and microstructure. AC and DC electrochemical tests were used to evaluate the effect of (i) organic additives (amino acids, proteins, vitamins, and antibiotics) in alpha-minimum essential medium (α-MEM) on electrochemical stability of noncoated and PEO-coated Ti and (ii) coating composition, microstructure, and corrosion behavior on the cell response in α-MEM. PEO-coated Ti showed higher corrosion resistance than the noncoated Ti in MEM with and without organic additives by an order of magnitude. The corrosion resistance in α-MEM decreased with time for nonmodified Ti and increased for PEO-coated Ti; the latter was because of the adsorption of the proteins in the coating pores which increased the diffusion resistance. The presence of Ca and P in titanium oxide coating at the Ca/P ratio exceeding that of any stoichiometric Ca-P-O and Ca-P-O-H compounds facilitates faster osteoblast cell adhesion.
采用等离子体电解氧化（PEO）工艺在钛上制备生物活性涂层。制备了钙/磷原子比为1.7和4.0的PEO涂层，并对其形貌、成分和微观结构进行了表征。采用交流和直流电化学测试来评估（i）α-最低基本培养基（α-MEM）中的有机添加剂（氨基酸、蛋白质、维生素和抗生素）对未涂层和PEO涂层钛的电化学稳定性的影响，以及（ii）涂层成分、微观结构和腐蚀行为对α-MEM中细胞反应的影响。在含有和不含有有机添加剂的MEM中，PEO涂层钛的耐腐蚀性比未涂层钛高一个数量级。对于未改性钛，α-MEM中的耐腐蚀性随时间降低，而对于PEO涂层钛则增加；后者是由于涂层孔隙中蛋白质的吸附增加了扩散阻力。氧化钛涂层中钙和磷的存在，其钙/磷比超过任何化学计量的Ca-P-O和Ca-P-O-H化合物，有利于成骨细胞更快地粘附。
