Departamento de Ingenieria Química y de Materiales, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain; Tissue Engineering Group, Institute of Biofunctional Studies (IEB-UCM), Associated Unit to the Institute of Polymer Science and Technology (CSIC), Polymer Functionalization Group, 28040 Madrid, Spain.
Departamento de Ingenieria Química y de Materiales, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain.
Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:738-752. doi: 10.1016/j.msec.2018.12.097. Epub 2018 Dec 28.
Bioactive PEO (Plasma Electrolytic Oxidation) coatings were generated on Grade I commercially pure titanium for dentistry applications using a Ca/P-based electrolyte with added Si, Mg, Zn or F species. Surface characteristics, chemical composition and ion liberation of the coatings were characterized using SEM/EDS, X-ray diffraction, optical profilometry, contact angle and ICP-OES. Corrosion resistance (OCP and DC polarization) was evaluated in SBF. Osteoblastogenesis and osteoclastogenesis processes on PEO-coated Ti and non-coated Ti controls were assessed after 7 days and 5 days of cell culture, respectively. Monolayer formation and metabolic activity were evaluated for the MC3T3 preosteoblastic cell line. All PEO coatings favoured differentiation processes over proliferation and presented three times greater quantity of secreted collagen than non-coated Ti control. All coating enabled osteoclast differentiation, with differences in number and size of the osteoclasts between the materials.
生物活性 PEO（等离子体电解氧化）涂层被应用于牙科领域的 I 级商用纯钛，使用基于 Ca/P 的电解质，添加 Si、Mg、Zn 或 F 物质。使用 SEM/EDS、X 射线衍射、光学轮廓仪、接触角和 ICP-OES 对涂层的表面特性、化学成分和离子释放进行了表征。在 SBF 中评估了耐腐蚀性（OCP 和 DC 极化）。在细胞培养 7 天和 5 天后，分别评估了 PEO 涂层钛和非涂层钛对照物上成骨细胞和破骨细胞的形成过程。MC3T3 前成骨细胞系评估了单层形成和代谢活性。所有 PEO 涂层都促进了分化过程，而不是增殖过程，并且分泌的胶原蛋白比非涂层钛对照物多三倍。所有涂层都能促进破骨细胞分化，不同材料之间破骨细胞的数量和大小存在差异。
