Zhao Xiu-Jun, Guo Yin, Song Qing-Liang, Li Li, Jiang Yan, Sun Yi-Chong, Qu Yin-E, Gao Fu-Lu
Department of Histology and Embryology, Hebei Medical University, Shijiazhuang 050017, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 Mar;44(2):205-8.
To explore the effect of Heshouwuyin on the expression of cytochrome C oxidase7a2 (Cox7a2) in testis tissue of rats with exercised-induced fatigue.
Fifty SD rats were divided into normal control group (A group), Heshouwuyin administered normal group (B group), model control group (C group), Heshouwuyin treated group (D group) and Heshouwuyin prevented group (E group) randomly with 10 rats for each. The exercise-induced fatigue models in rats of C, D, E groups were established. The rats in D group were treated with Heshouwuyin [20 g/(kg x d), contained crude drug 9.6 g/mL] for 60 days (during the 42 days of modeling and after the 18 days of modeling). The rats in E group were also treated with Heshouwuyin for 60 days (but before the 18 days of modeling and during the 42 days of modeling). Beckmancoulter Unicel Dxl 800 was used to detect the level of serum testosterone, according to the manufacture's instructions. Western blot and RT-PCR were used to observe the differential expression of Cox7a2.
The level of serum testosterone in C group was decreased compared with A group (P < 0.05), which implied the success of modeling. Compared with group A, the level of serum testosterone in B, D, E groups were increased (P < 0.05). Cox7a2 protein was expressed mainly in leydig cell and spermatocyte. Compared with A,B, D, E groups, the expression of Cox7a2 protein and mRNA in C group increased (P < 0.05), and there no significant difference was observed between group A and B, as well as group D and E.
The expression of Cox7a2 was down-regulated by Heshouwuyin.
探讨何首乌饮对运动性疲劳大鼠睾丸组织中细胞色素C氧化酶7a2（Cox7a2）表达的影响。
将50只SD大鼠随机分为正常对照组（A组）、何首乌饮灌胃正常组（B组）、模型对照组（C组）、何首乌饮治疗组（D组）和何首乌饮预防组（E组），每组10只。建立C、D、E组大鼠运动性疲劳模型。D组大鼠给予何首乌饮[20 g/(kg·d)，含生药9.6 g/mL]灌胃60天（造模42天期间及造模18天后）。E组大鼠也给予何首乌饮灌胃60天（但在造模18天前及造模42天期间）。按照仪器说明书，采用贝克曼库尔特Unicel Dxl 800检测血清睾酮水平。采用蛋白质免疫印迹法（Western blot）和逆转录-聚合酶链反应（RT-PCR）观察Cox7a2的差异表达。
与A组比较，C组血清睾酮水平降低（P<0.05），提示造模成功。与A组比较，B、D、E组血清睾酮水平升高（P<0.05）。Cox7a2蛋白主要表达于睾丸间质细胞和精母细胞。与A、B、D、E组比较，C组Cox7a2蛋白及mRNA表达增加（P<0.05），A组与B组、D组与E组比较差异无统计学意义。
何首乌饮可下调Cox7a2的表达。
