Bilousov O O, Katanaev V L, Demydov S V, Kozeretska I A
Department of Pharmacology and Toxicology, University of Lausanne, Switzerland.
Tsitol Genet. 2013 Mar-Apr;47(2):77-81.
During maturation Drosophila wing epithelial cells undergo number of changes due to processes, which take place in the wing of the newly emerged fly, among which epithelial-to-mesenchymal transition (EMT) and apoptosis are pivotal. It is considered that neurohormone bursicon is responsible for their triggering. In turn, extracellular matrix protein Miniature is also essential for proper progress of apoptosis and, presumably, EMT. In accordance with our previously proposed hypothesis, Miniature and bursicon form stabilizing/accumulative complexes, which are able to diffuse freely within Drosophila wing, in such a way constitutively promoting enough concentrations of the maturation triggering signal. Here we tried to come to confirmation of our hypothesis from the other side, using UAS/GAL4 system and RNAi-silencing techniques.
在果蝇翅膀成熟过程中，翅膀上皮细胞会因新羽化果蝇翅膀中发生的一系列过程而经历多种变化，其中上皮-间质转化（EMT）和细胞凋亡至关重要。据认为，神经激素bursicon负责触发这些过程。反过来，细胞外基质蛋白Miniature对于细胞凋亡的正常进行以及可能的EMT也至关重要。根据我们之前提出的假设，Miniature和bursicon形成稳定/积累复合物，这些复合物能够在果蝇翅膀内自由扩散，从而持续促进足够浓度的成熟触发信号。在这里，我们试图从另一个角度证实我们的假设，使用UAS/GAL4系统和RNA干扰沉默技术。
