Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Environ Sci Technol. 2013 Jul 2;47(13):7446-56. doi: 10.1021/es401639x. Epub 2013 Jun 21.
Polycyclic aromatic hydrocarbons (PAHs) are known to induce reactive oxygen species and oxidative stress, but the dose-response relationships between exposure to PAHs and oxidative stress levels have not been established. In this study, we recruited 1333 male coke oven workers, monitored the levels of environmental PAHs, and measured internal PAH exposure biomarkers including 12 urinary PAH metabolites and plasma benzo[a]pyrene-r-7,t-8,t-9,c-10-tetrahydotetrol-albumin (BPDE-Alb) adducts, as well as the two oxidative biomarkers urinary 8-hydroxydeoxyguanosine (8-OHdG) and 8-iso-prostaglandin-F2α (8-iso-PGF2α). We found that the total concentration of urinary PAH metabolites and plasma BPDE-Alb adducts were both significantly associated with increased 8-OHdG and 8-iso-PGF2α in both smokers and nonsmokers (all p < 0.05). This exposure-response effect was also observed for most PAH metabolites (all p(trend) < 0.01), except for 4-hydroxyphenanthrene and 8-OHdG (p(trend) = 0.108). Furthermore, it was shown that only urinary 1-hydroxypyrene has a significant positive association with both 8-OHdG and 8-iso-PGF2α after a Bonferroni correction (p < 0.005). Our results indicated that urinary ΣOH-PAHs and plasma BPDE-Alb adducts can result in significant dose-related increases in oxidative damage to DNA and lipids. Furthermore, when a multianalyte method is unavailable, our findings demonstrate that urinary 1-hydroxypyrene is a useful biomarker for evaluating total PAHs exposure and assessing oxidative damage in coke oven workers.
多环芳烃（PAHs）已知会诱导活性氧和氧化应激，但环境中 PAHs 暴露与氧化应激水平之间的剂量-反应关系尚未确定。在这项研究中，我们招募了 1333 名男性焦炉工人，监测环境 PAHs 水平，并测量了包括 12 种尿液 PAH 代谢物和血浆苯并[a]芘-r-7,t-8,t-9,c-10-四氢二氢四醇-白蛋白（BPDE-Alb）加合物在内的内部 PAH 暴露生物标志物，以及尿液 8-羟基脱氧鸟苷（8-OHdG）和 8-异前列腺素-F2α（8-iso-PGF2α）这两个氧化生物标志物。我们发现，尿液中 PAH 代谢物的总浓度和血浆中 BPDE-Alb 加合物的浓度都与吸烟者和非吸烟者的 8-OHdG 和 8-iso-PGF2α 水平升高显著相关（所有 p<0.05）。对于大多数 PAH 代谢物（所有 p(trend)<0.01），除了 4-羟基菲和 8-OHdG（p(trend)=0.108），都观察到这种暴露-反应效应。此外，在经过 Bonferroni 校正后，只有尿液中 1-羟基芘与 8-OHdG 和 8-iso-PGF2α 都有显著的正相关关系（p<0.005）。我们的研究结果表明，尿液中ΣOH-PAHs 和血浆中 BPDE-Alb 加合物可导致 DNA 和脂质的氧化损伤与剂量呈显著相关。此外，当无法使用多分析物方法时，我们的研究结果表明，尿液中 1-羟基芘是评估焦炉工人总 PAHs 暴露和评估氧化损伤的有用生物标志物。
