Department of Physiology, The University of Auckland, Auckland, New Zealand.
Clin Exp Pharmacol Physiol. 2013 Sep;40(9):671-81. doi: 10.1111/1440-1681.12139.
Herein we describe our laboratories' experimental methods for interrogating cardiac energetics at the organ (whole heart), tissue (trabecula) and perforated fibre (mitochondrial) levels. In whole heart and trabecula experiments, we focus on measuring pressure-volume (force-length) work and oxygen consumption (heat production) from which mechanical efficiency is derived. In both preparations (i.e. across scales differing by three orders of magnitude) we find efficiency values of 10%-15%. Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron leak and reactive oxygen species production, the latter two of which index energy transfer inefficiencies.
在此，我们描述了实验室在器官（整个心脏）、组织（心肌小梁）和穿孔纤维（线粒体）水平研究心脏能量学的实验方法。在整个心脏和心肌小梁实验中，我们专注于测量压力-容积（力-长度）功和耗氧量（产热量），从而得出机械效率。在这两种制备物中（即在相差三个数量级的尺度上），我们发现效率值为 10%-15%。线粒体实验采用了三组滴定方案来提供关于耗氧量、ATP 通量、膜电位、电子泄漏和活性氧物质产生的信息，后两者指标能量传递效率低下。
