College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
Anal Chim Acta. 2013 Jun 19;784:47-52. doi: 10.1016/j.aca.2013.05.002. Epub 2013 May 13.
In this paper, we report a double-receptor sandwich type fluorescence sensing method for the determination of fructose bisphosphates (FBPs) using fructose 1,6-bisphosphate (F-1,6-BP) as a model analyte based on uranyl-salophen complexes. The solid phase receptor is an immobilized uranyl-salophen (IUS) complex which is bound on the surface of glass slides by covalent bonds. The labeled receptor is another uranyl-salophen complex containing a fluorescence group, or uranyl-salophen-fluorescein (USF). In the procedure of determining F-1,6-BP in sample solution, F-1,6-BP is first adsorbed on the surface of the glass slide through the coordination reaction of F-1,6-BP with IUS. It then binds USF through another coordination reaction to form a sandwich-type structure of IUS-F-1,6-BP-USF. The amount of F-1,6-BP is detected by the determination of the fluorescence intensity of IUS-F-1,6-BP-USF bound on the glass slide. Under optimal conditions, the linear range for the detection of F-1,6-BP is 0.05-5.0 nmol mL(-1) with a detection limit of 0.027 nmol mL(-1). The proposed method has been successfully applied for the determination of F-1,6-BP in real samples with satisfactory results.
本文报道了一种基于铀-席夫碱配合物的双受体夹心型荧光传感方法，用于测定果糖-1,6-二磷酸（FBP）。以果糖-1,6-二磷酸（F-1,6-BP）为模型分析物，该方法使用了果糖 1,6-双磷酸（F-1,6-BP）。固相受体是通过共价键结合在载玻片表面的固定化铀-席夫碱（IUS）配合物。标记受体是另一种含有荧光团的铀-席夫碱（USF）配合物。在测定样品溶液中 F-1,6-BP 的过程中，F-1,6-BP 通过 F-1,6-BP 与 IUS 的配位反应首先被吸附在载玻片表面上。然后，它通过另一个配位反应与 USF 结合，形成 IUS-F-1,6-BP-USF 的夹心型结构。通过测定结合在载玻片上的 IUS-F-1,6-BP-USF 的荧光强度来检测 F-1,6-BP 的含量。在最佳条件下，F-1,6-BP 的检测线性范围为 0.05-5.0 nmol·mL-1，检测限为 0.027 nmol·mL-1。该方法已成功应用于实际样品中 F-1,6-BP 的测定，结果令人满意。
