College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
Anal Chim Acta. 2012 Jun 4;729:80-4. doi: 10.1016/j.aca.2012.04.012. Epub 2012 Apr 20.
A double-receptor sandwich supramolecule method for the separation and determination of trace uranium was proposed in this paper. One receptor is a salophen which can react with uranyl to form a uranyl-salophen complex, and another receptor is an oligonucleotide which can bind uranyl to form oligonucleotide-uranyl-salophen supramolecule. The salophen was immobilized on the surface of silica gel particles and used as the solid phase receptor for separating uranium from solution. The oligonucleotide was labeled with a fluorescent group and used as the labeled receptor for quantitatively analyzing uranium. In the procedure of separation and determination, uranyl ion was first combined with the solid phase receptor and then conjugated with the labeled receptor to form the sandwich-type supramolecule. The labeled receptor in the sandwich supramolecule was then eluted and determined by fluorescence analysis. The experimental results demonstrate that this method has a number of advantages such as high selectivity, excellent pre-concentration capability, high sensitivity, good stability and low cost. Under optimal conditions, the linear range for the detection of uranium is 0.5-30.0 ng mL(-1) with a detection limit of 0.2 ng mL(-1). The proposed method was successfully applied for the separation and determination of uranium in real samples with the recoveries of 95.0-105.5%.
本文提出了一种用于痕量铀分离和测定的双受体夹心超分子方法。其中一个受体是 salophen，它可以与铀酰反应形成铀酰-salophen 配合物，另一个受体是寡核苷酸，它可以与铀酰结合形成寡核苷酸-铀酰-salophen 超分子。salophen 固定在硅胶颗粒表面上作为固相受体，用于从溶液中分离铀。寡核苷酸被标记上荧光基团作为标记受体，用于定量分析铀。在分离和测定过程中，铀酰离子首先与固相受体结合，然后与标记受体结合形成夹心型超分子。然后通过荧光分析洗脱和测定夹心超分子中的标记受体。实验结果表明，该方法具有高选择性、优异的预富集能力、高灵敏度、良好的稳定性和低成本等优点。在最佳条件下，铀的检测线性范围为 0.5-30.0ng/mL，检测限为 0.2ng/mL。该方法成功地应用于实际样品中铀的分离和测定，回收率为 95.0-105.5%。
