Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
Department of Chemical Engineering, Yancheng Institute of Industry Technology, Yancheng, Jiangsu, China.
Biosens Bioelectron. 2014 Aug 15;58:266-71. doi: 10.1016/j.bios.2014.02.068. Epub 2014 Mar 7.
Oligonucleotide-templated Ag nanoclusters (DNA-Ag NCs) prepared from AgNO3 using an oligonucleotide (5'-TAACCCCTAACCCCT-3') as a template and NaBH4 as a reducing agent have been used for sensing of adenosine 5'-triphosphate (ATP). The fluorescence intensity and emission wavelength of DNA-Ag NCs are dependent on the pH value and ATP concentration. At pH 3.0 and 11.0, ATP shows greater effects on fluorescence of the DNA-Ag NCs. Upon increasing ATP concentration from 10 to 50μM, their emission wavelength at pH 3.0 shifts from 525 to 585nm. At pH 11.0, their fluorescence intensity (510nm) increases upon increasing ATP concentration. The circular dichroism (CD), electrospray ionization-mass spectrometry (ESI-MS), absorption, and fluorescence results indicate that ATP and pH affect the interactions between DNAs and Ag atoms, resulting in changes in their fluorescence. The DNA-Ag NCs allow detection of ATP over a concentration range of 0.1-10μM, with a limit of detection 33nM. Practicality of the DNA-Ag NCs probe has been validated with the determination of ATP concentrations in the lysate of MDA-MB-231 breast carcinoma cells.
寡核苷酸模板 Ag 纳米团簇（DNA-Ag NCs）是由 AgNO3 用寡核苷酸（5'-TAACCCCTAACCCCT-3'）作为模板和 NaBH4 作为还原剂制备的，用于检测腺苷 5'-三磷酸（ATP）。DNA-Ag NCs 的荧光强度和发射波长取决于 pH 值和 ATP 浓度。在 pH 值为 3.0 和 11.0 时，ATP 对 DNA-Ag NCs 的荧光具有更大的影响。随着 ATP 浓度从 10 增加到 50μM，其在 pH 值为 3.0 时的发射波长从 525nm 移至 585nm。在 pH 值为 11.0 时，其荧光强度（510nm）随 ATP 浓度的增加而增加。圆二色性（CD）、电喷雾电离质谱（ESI-MS）、吸收和荧光结果表明，ATP 和 pH 值影响 DNA 与 Ag 原子之间的相互作用，导致其荧光发生变化。DNA-Ag NCs 允许在 0.1-10μM 的浓度范围内检测 ATP，检测限为 33nM。通过测定 MDA-MB-231 乳腺癌细胞裂解物中的 ATP 浓度，验证了 DNA-Ag NCs 探针的实用性。
