Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR 7104, INSERM U 964, Université de Strasbourg, 67404 Illkirch, France.
Cell Rep. 2013 Jun 27;3(6):2142-54. doi: 10.1016/j.celrep.2013.05.003. Epub 2013 Jun 6.
Human cells contain five canonical, replication-dependent somatic histone H1 subtypes (H1.1, H1.2, H1.3, H1.4, and H1.5). Although they are key chromatin components, the genomic distribution of the H1 subtypes is still unknown, and their role in chromatin processes has thus far remained elusive. Here, we map the genomic localization of all somatic replication-dependent H1 subtypes in human lung fibroblasts using an integrative DNA adenine methyltransferase identification (DamID) analysis. We find in general that H1.2 to H1.5 are depleted from CpG-dense regions and active regulatory regions. H1.1 shows a DamID binding profile distinct from the other subtypes, suggesting a unique function. H1 subtypes can mark specific domains and repressive regions, pointing toward a role for H1 in three-dimensional genome organization. Our work integrates H1 subtypes into the epigenome maps of human cells and provides a valuable resource to refine our understanding of the significance of H1 and its heterogeneity in the control of genome function.
人类细胞含有五种典型的、依赖于复制的体细胞核小体组蛋白 H1 亚型（H1.1、H1.2、H1.3、H1.4 和 H1.5）。尽管它们是染色质的关键组成部分，但 H1 亚型在基因组上的分布仍不清楚，其在染色质过程中的作用也一直难以捉摸。在这里，我们使用整合的 DNA 腺嘌呤甲基转移酶鉴定（DamID）分析来绘制人肺成纤维细胞中所有依赖于复制的体细胞 H1 亚型的基因组定位。我们发现，H1.2 到 H1.5 通常从 CpG 密集区域和活性调控区域中缺失。H1.1 显示出与其他亚型不同的 DamID 结合谱，表明其具有独特的功能。H1 亚型可以标记特定的结构域和抑制区域，这表明 H1 在三维基因组组织中具有一定的作用。我们的工作将 H1 亚型整合到人类细胞的表观基因组图谱中，为我们深入了解 H1 的意义及其在控制基因组功能方面的异质性提供了宝贵的资源。
