Wang X L, Liang J Y, Liu R Z, Dong Z, Yu S Y
Department of Neurology, Chinese PLA General Hospital, Beijing 100853, China.
Neuroscience. 2013 Sep 17;248:145-53. doi: 10.1016/j.neuroscience.2013.05.028. Epub 2013 Jun 5.
Using two-dimensional gel electrophoresis (2-DE), we examined proteomic changes in the caudal part of the spinal trigeminal nucleus induced by electrical stimulation of the dura mater surrounding the superior sagittal sinus (ES-SSS) in conscious rats. After image analysis of 2-DE gels, nine protein spots of interest were excised from the gels and identified by liquid chromatography-tandem mass spectrometry. Among the nine, succinate semialdehyde dehydrogenase (SSADH) was found to be down-regulated after ES-SSS. This result was validated with Real-time polymerase chain reaction and Western blot analyses. Because SSADH degrades GABA, decreased expression of it increases the local concentration of GABA in the caudal part of the spinal trigeminal nucleus after ES-SSS; this has not been reported before and may participate in the modulation of trigeminovascular headache.
我们使用二维凝胶电泳（2-DE）技术，研究了在清醒大鼠中，电刺激上矢状窦周围硬脑膜（ES-SSS）后，三叉神经脊束核尾部的蛋白质组学变化。对2-DE凝胶进行图像分析后，从凝胶中切下9个感兴趣的蛋白点，并通过液相色谱-串联质谱法进行鉴定。在这9个蛋白点中，发现琥珀酸半醛脱氢酶（SSADH）在ES-SSS后表达下调。这一结果通过实时聚合酶链反应和蛋白质印迹分析得到验证。由于SSADH可降解γ-氨基丁酸（GABA），其表达降低会使ES-SSS后三叉神经脊束核尾部的GABA局部浓度升高；这一现象此前未见报道，可能参与了三叉神经血管性头痛的调节。
