Jiang Lei, Dong Zhao, Li Fengpeng, Liu Ruozhuo, Qiu Enchao, Wang Xiaolin, Yu Shengyuan
Department of Neurology, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Department of Neurology, Chinese People's Liberation Army General Hospital, Beijing 100853, China. Email:
Chin Med J (Engl). 2014;127(4):734-41.
The molecular and cellular origins of migraine headache are among the most complex problems in contemporary neurology. Up to now the pathogenesis of migraine still remains unclearly defined. The objective of this study was to explore new factors that may be related to the mechanism of migraine.
The present study performed a comprehensive analysis of gene expression in the trigeminal nucleus caudalis induced by electrical stimulation of dura mater surrounding the superior sagittal sinus in conscious rats using microarray analysis followed by quantitative real-time reverse-transcribed polymerase chain reaction (qRT-PCR) verification. Student's two sample t-test was employed when two groups were compared. A P value <0.05 was considered to be statistically significant.
Comparing the placebo and the electrical stimulation groups, 40 genes were determined to be significantly differentially expressed. These significantly differentially expressed genes were involved in many pathways, including transporter activity, tryptophan metabolism, G protein signaling, kinase activity, actin binding, signal transducer activity, anion transport, protein folding, enzyme inhibitor activity, coenzyme metabolism, binding, ion transport, cell adhesion, metal ion transport, oxidoreductase activity, mitochondrion function, and others. Most of the genes were involved in more than 2 pathways. Of particular interest is the up-regulation of Phactr3 and Akap5 and the down-regulation of Kdr.
These findings may provide important clues for a better understanding of the molecular mechanism of migraine.
偏头痛的分子和细胞起源是当代神经学中最复杂的问题之一。迄今为止，偏头痛的发病机制仍未明确界定。本研究的目的是探索可能与偏头痛机制相关的新因素。
本研究采用微阵列分析，随后进行定量实时逆转录聚合酶链反应（qRT-PCR）验证，对清醒大鼠上矢状窦周围硬脑膜电刺激诱导的三叉神经尾核中的基因表达进行了综合分析。比较两组时采用学生双样本t检验。P值<0.05被认为具有统计学意义。
比较安慰剂组和电刺激组，确定有40个基因存在显著差异表达。这些显著差异表达的基因涉及许多途径，包括转运体活性、色氨酸代谢、G蛋白信号传导、激酶活性、肌动蛋白结合、信号转导活性、阴离子转运、蛋白质折叠、酶抑制活性、辅酶代谢、结合、离子转运、细胞粘附、金属离子转运、氧化还原酶活性、线粒体功能等。大多数基因涉及不止2条途径。特别值得关注的是Phactr3和Akap5的上调以及Kdr的下调。
这些发现可能为更好地理解偏头痛的分子机制提供重要线索。
