CytoGen, Songpa-gu, Seoul 138-961, Republic of Korea.
Anal Biochem. 2013 Sep 1;440(1):114-6. doi: 10.1016/j.ab.2013.05.016. Epub 2013 Jun 4.
Enrichment of circulating tumor cells (CTCs) from whole blood is very challenging due to its rarity. We have developed a new CTC enrichment method using a microfabricated filter. The filter was designed to fractionate tumor cells by cell size and optimized to have high porosity and proper pore distribution. When cancer cells were spiked in whole blood, the average recovery rate was 82.0 to 86.7% and the limit of detection by filtration process was approximately 2 cancer cells in a testing volume of blood. The results indicate that the microfabricated filter-based enrichment would be useful to retrieve and analyze CTCs in practice.
从全血中富集循环肿瘤细胞（CTCs）非常具有挑战性，因为其非常罕见。我们开发了一种使用微制造过滤器富集 CTC 的新方法。该过滤器旨在通过细胞大小对肿瘤细胞进行分级，并经过优化，使其具有高孔隙率和适当的孔分布。当癌细胞被掺入全血时，平均回收率为 82.0%至 86.7%，通过过滤过程的检测限约为测试体积血液中的 2 个癌细胞。结果表明，基于微制造过滤器的富集方法将有助于在实际中检索和分析 CTC。
