Department of Animal Biotechnology, College of Animal Life and Science, Kangwon National University, Chuncheon 200-701, South Korea.
Biochem Biophys Res Commun. 2013 Jul 5;436(3):413-7. doi: 10.1016/j.bbrc.2013.05.116. Epub 2013 Jun 6.
Poor understanding of the differentiation of mesenchymal stem cells (MSCs) has resulted in a low differentiation yield, and has hindered their application in medicine. As a solution, priming MSCs sensitive to signaling, thus stimulating differentiation into a specific cell lineage, may improve the differentiation yield. To demonstrate this, priming MSCs were produced by using a gelatin matrix for the isolation of primary MSCs from bone-marrow-derived primary cells. Subsequently, cellular characteristics and sensitivity to specific differentiation signals were analyzed at passage five. Compared to non-priming MSCs, priming MSCs showed no significant differences in cellular characteristics, but demonstrated a significant increase in sensitivity to neurogenic differentiation signals. These results demonstrate that generation of priming MSCs by specific extracellular signaling increases the rate of differentiation into a cell-specific lineage.
对间充质干细胞（MSCs）分化的认识不足导致其分化率低，限制了其在医学中的应用。作为解决方案，通过预先刺激 MSCs 对信号敏感，从而刺激其分化为特定的细胞谱系，可能会提高分化率。为了证明这一点，使用明胶基质从骨髓来源的原代细胞中分离原代 MSCs 来制备预刺激 MSCs。随后，在第 5 代时分析细胞特征和对特定分化信号的敏感性。与非预刺激 MSCs 相比，预刺激 MSCs 的细胞特征没有显著差异，但对神经发生分化信号的敏感性显著增加。这些结果表明，通过特定的细胞外信号生成预刺激 MSCs 可提高向特定细胞谱系分化的速度。
