Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390 002, Gujarat, India.
Bioresour Technol. 2013 Aug;142:436-44. doi: 10.1016/j.biortech.2013.05.057. Epub 2013 May 22.
Reactive Violet 5R (RV5R) decolorising acclimatised bacterial consortia were enriched from industrial effluent contaminated and pristine samples from Gujarat, India on several different media. Twelve acclimatised consortia were selected for the study which were able to decolorise 100mg/L RV5R in 30 h under shaking or static conditions. Eubacterial diversity was studied by 16S rRNA gene based culture-independent methods, using HaeIII and Hinf1 enzymes for ARDRA and V3 region based DGGE analysis, forming total 6 clusters in both analysis. Decolorised end products of all the consortia were analysed by FTIR showing cleavage of the azo bond and group modifications. GC-MS data of dye decolorised end products of Gly consortium obtained from hydrocarbon contaminated soil demonstrated benzene ring cleavage activity. Present study suggests that enrichment of acclimatised consortia under different conditions can result in diverse microbial communities that differentially degrade RV5R and can provide rich source of dye decolorising strains.
从印度古吉拉特邦受工业废水污染和原始样本中，利用几种不同的培养基，富集了能适应环境的、能将活性艳红 5R（RV5R）脱色的细菌混合菌群。本研究选择了 12 种能在振荡或静置条件下 30 小时内将 100mg/L RV5R 脱色的适应菌群。通过基于 16S rRNA 基因的非培养依赖方法研究了真细菌多样性，使用 HaeIII 和 Hinf1 酶进行 ARDRA 分析，以及基于 V3 区的 DGGE 分析，这两种分析共形成了 6 个聚类。所有菌群的脱色终产物都通过傅里叶变换红外光谱（FTIR）进行了分析，结果表明偶氮键被切断，基团发生了修饰。从烃类污染土壤中获得的 Gly 菌群对染料脱色终产物的 GC-MS 数据分析表明，该菌群具有苯环断裂活性。本研究表明，在不同条件下富集适应菌群会导致不同的微生物群落，这些群落能够不同程度地降解 RV5R，为染料脱色菌株提供了丰富的来源。
