Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
Methods. 2013 Dec 15;64(3):224-8. doi: 10.1016/j.ymeth.2013.05.017. Epub 2013 Jun 2.
We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.
我们在此报告了一种基于发光开关的无标签 G-四链体测定法，用于快速灵敏地检测聚合酶校对活性，使用新型铱（III）配合物作为 G-四链体选择性探针。铱（III）配合物与 G-四链体基序的相互作用促进了聚合酶校对活性的高灵敏度开关检测。使用 T4 DNA 聚合酶（T4 pol）作为模型酶，该测定法对 T4 pol 具有高灵敏度和选择性，优于其他测试酶。
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