使用G-四链体选择性探针实现对T4多核苷酸激酶活性的无标记发光开启检测。
Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe.
作者信息
He Hong-Zhang, Leung Ka-Ho, Wang Wei, Chan Daniel Shiu-Hin, Leung Chung-Hang, Ma Dik-Lung
机构信息
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
出版信息
Chem Commun (Camb). 2014 May 25;50(40):5313-5. doi: 10.1039/c3cc47444e. Epub 2013 Dec 16.
A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.
利用一种新型的G-四链体选择性发光铱（III）配合物探针，开发了一种用于检测T4多核苷酸激酶活性的无标记、基于寡核苷酸的开启式发光检测方法。还展示了该检测方法在筛选潜在T4 PNK抑制剂方面的应用。据我们所知，这是首个基于金属的PNK活性检测方法。
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