用于检测纳摩尔钙离子(II)的平行 G-四链体选择性发光探针。
A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion.
机构信息
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
出版信息
Methods. 2013 Dec 15;64(3):212-7. doi: 10.1016/j.ymeth.2013.07.021. Epub 2013 Jul 19.
A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca(2+) ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G4T4G4-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca(2+) ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca(2+) ions with a limit of detection in the nanomolar range, and was selective for Ca(2+) over other metal ions.
已合成了一种平行 G-四链体选择性铱(III)配合物，并将其用作在水溶液中用于检测 Ca(2+)离子的无标记 G-四链体检测分析中的荧光探针。在该分析中，富含鸟嘌呤的寡核苷酸（G4，5'-G4T4G4-3'）最初以反平行 G-四链体构象存在，导致低的荧光信号。与 Ca(2+)离子孵育后，反平行 G-四链体被诱导形成平行 G-四链体构象，这大大增强了铱(III)探针的荧光发射。该方法对 Ca(2+)离子具有很高的灵敏度，检测限在纳摩尔范围内，并且对 Ca(2+)具有选择性，而对其他金属离子则没有选择性。
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