Lin Na, Chen Si, Zhang Hong, Li Junmin, Fu Linglin
Key Laboratory of Mariculture & Enhancement, Marine Fisheries Research Institute of Zhejiang Province , Zhoushan 316000, China.
Marine and Fisheries Research Institute, Zhejiang Ocean University , Zhoushan, Zhejiang 316000, China.
J Agric Food Chem. 2018 Feb 7;66(5):1270-1278. doi: 10.1021/acs.jafc.7b05698. Epub 2018 Jan 30.
Major royal jelly protein 1 (MRJP1) is the most abundant protein in royal jelly (RJ), and the level of MRJP1 has been suggested as a promising parameter for standardization and evaluation of RJ authenticity in quality. Here, a quantitative method was developed for the quantification of MRJP1 in RJ based on a signature peptide and a stable isotope-labeled internal standard peptide FFDYDFGSDER*(R*, C, N) by ultraperformance liquid chromatography-tandem mass spectrometry. Recoveries of the established method ranged from 85.33 to 95.80%, and both the intra- and interday precision were RSD < 4.97%. Quantification results showed that content of MRJP1 in fresh RJ was 41.96-55.01 mg/g. Abnormal levels of MRJP1 were found in three commercial RJs and implied that these samples were of low quality and might be adulterated. Results of the present work suggested that the developed method could be successfully applied to quantify MRJP1 in RJ and also could evaluate the quality of RJ.
主要蜂王浆蛋白1（MRJP1）是蜂王浆（RJ）中含量最丰富的蛋白质，MRJP1的含量已被认为是质量标准化和评估RJ真伪的一个有前景的参数。在此，基于一个特征肽和一个稳定同位素标记的内标肽FFDYDFGSDER*(R*, C, N)，开发了一种通过超高效液相色谱-串联质谱法定量RJ中MRJP1的方法。所建立方法的回收率在85.33%至95.80%之间，日内和日间精密度的相对标准偏差均<4.97%。定量结果表明，新鲜RJ中MRJP1的含量为41.96 - 55.01 mg/g。在三种市售RJ中发现MRJP1水平异常，这意味着这些样品质量较低且可能掺假。本研究结果表明，所开发的方法可成功应用于定量RJ中的MRJP1，也可评估RJ的质量。
