细胞色素P450 BM3变体F87V催化生成羟基化黄酮类化合物及其抗氧化活性。
Production of hydroxlated flavonoids with cytochrome P450 BM3 variant F87V and their antioxidative activities.
作者信息
Kitamura Emi, Otomatsu Toshihiko, Maeda Chiemi, Aoki Yoko, Ota Chihiro, Misawa Norihiko, Shindo Kazutoshi
机构信息
Department of Food and Nutrition, Japan Women's University, Tokyo, Japan.
出版信息
Biosci Biotechnol Biochem. 2013;77(6):1340-3. doi: 10.1271/bbb.130148. Epub 2013 Jun 7.
A variant of P450 BM3 with an F87V substitution [P450 BM3 (F87V)] is a substrate-promiscuous cytochrome P450 monooxygenase. We investigated the bioconversion of various flavonoids (favanones, chalcone, and isoflavone) by using recombinant Escherichia coli cells, which expressed the gene coding for P450 BM3 (F87V), to give their corresponding hydroxylated products. Potent antioxidative activities were observed in some of the products.
一种具有F87V替代的P450 BM3变体[P450 BM3 (F87V)]是一种底物混杂的细胞色素P450单加氧酶。我们利用表达编码P450 BM3 (F87V)的基因的重组大肠杆菌细胞，研究了各种黄酮类化合物（黄烷酮、查耳酮和异黄酮）的生物转化，以生成它们相应的羟基化产物。在一些产物中观察到了强大的抗氧化活性。
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