Department of Physics, Annamalai University, Annamalainagar, Chidambaram, 608 002, Tamilnadu, India,
Mol Cell Biochem. 2013 Oct;382(1-2):27-36. doi: 10.1007/s11010-013-1715-6. Epub 2013 Jun 9.
The aim of the present study is to investigate the chemopreventive effects of the prepared naringenin-loaded nanoparticles (NARNPs) relative to efficacy of free naringenin (NAR) in modifying the functional, structural, and compositional changes at the molecular level during 7, 12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis by Fourier transform infrared (FT-IR) spectroscopy. The results revealed that a significant increase in the amount of proteins and nucleic acid contents and a decrease in the amount of lipids and glycogen contents are observed in DMBA-induced tumor tissues. In addition, in tumor tissues a decrease in lipid order and a significant increase in membrane dynamics were noticed. Further, the composition and secondary structure of proteins were found to be altered, which indicates some important structural alterations in the existing proteins and/or the expression of new types of proteins occurring under the tumor transformation. Furthermore, oral administration of free NAR and NARNPs significantly increased lipids and their order as well as increased the glycogen contents and decreased the levels of proteins and nucleic acid contents. On a comparative basis, NARNPs were found to have a more potent antitumor effect than free NAR in completely preventing the formation of squamous cell carcinoma and in improving the biochemical constituents to a normal range in DMBA-induced HBP carcinogenesis. The present study further shows a great potential of FT-IR spectroscopy as a complimentary tool for the screening of various anticancer drugs and follow-up, which may allow faster response to critical problems arising during treatment.
本研究旨在探讨制备的柚皮素载药纳米粒（NARNPs）相对于游离柚皮素（NAR）在通过傅里叶变换红外（FT-IR）光谱研究 7,12-二甲基苯并[a]蒽（DMBA）诱导的颊囊（HBP）致癌过程中在分子水平上对功能、结构和组成变化的化学预防作用。结果表明，DMBA 诱导的肿瘤组织中蛋白质和核酸含量显著增加，脂质和糖原含量减少。此外，在肿瘤组织中观察到脂质有序性降低和膜动力学显著增加。此外，还发现蛋白质的组成和二级结构发生改变，这表明在肿瘤转化过程中存在的蛋白质发生了一些重要的结构改变和/或表达了新类型的蛋白质。此外，游离 NAR 和 NARNPs 的口服给药显著增加了脂质及其有序性，增加了糖原含量，并降低了蛋白质和核酸含量的水平。从比较的角度来看，NARNPs 比游离 NAR 更能有效地预防鳞状细胞癌的形成，并改善 DMBA 诱导的 HBP 癌变过程中的生化成分恢复到正常范围。本研究进一步表明，FT-IR 光谱作为筛选各种抗癌药物和随访的辅助工具具有很大的潜力，这可能允许对治疗过程中出现的关键问题更快地做出反应。
