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rubneri 链球菌,新种,从人体咽喉分离得到。

Streptococcus rubneri sp. nov., isolated from the human throat.

机构信息

Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Department of Safety and Quality of Fruit and Vegetables, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany.

Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.

出版信息

Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4026-4032. doi: 10.1099/ijs.0.048538-0. Epub 2013 Jun 7.

Abstract

The novel, Gram-stain-positive, ovoid, lactic acid bacterial isolates LMG 27205, LMG 27206, LMG 27207(T) and MRI-F 18 were obtained from throat samples of healthy humans. 16S rRNA gene sequence analyses indicated that these isolates belong to the genus Streptococcus, specifically the Streptococcus mitis group, with Streptococcus australis and Streptococcus mitis as the nearest neighbours (99.45 and 98.56 % 16S rRNA gene sequence similarity to the respective type strains). Genotypic fingerprinting by fluorescent amplified fragment length polymorphism (FAFLP) and pulsed-field gel electrophoresis (PFGE), DNA-DNA hybridizations, comparative sequence analysis of pheS, rpoA and atpA and physiological and biochemical tests revealed that these bacteria formed a taxon well separated from its nearest neighbours and other species of the genus Streptococcus with validly published names and, therefore, represent a novel species, for which the name Streptococcus rubneri sp. nov. is proposed, with LMG 27207(T) ( = DSM 26920(T)) as the type strain.

摘要

从健康人类的咽喉样本中获得了新型革兰氏阳性、卵圆形、乳酸菌分离株 LMG 27205、LMG 27206、LMG 27207(T) 和 MRI-F 18。16S rRNA 基因序列分析表明,这些分离株属于链球菌属,具体为缓症链球菌群,与口腔链球菌和米氏链球菌最为接近(与各自的模式株的 16S rRNA 基因序列相似度为 99.45%和 98.56%)。荧光扩增片段长度多态性(FAFLP)和脉冲场凝胶电泳(PFGE)、DNA-DNA 杂交、pheS、rpoA 和 atpA 的比较序列分析、生理生化试验的基因指纹图谱显示,这些细菌与最接近的近缘种和其他具有有效发表名称的链球菌属物种形成了一个分类群,因此代表了一个新种,建议使用 LMG 27207(T)(=DSM 26920(T))作为模式株,命名为红球菌属。

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