College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
Int J Syst Evol Microbiol. 2019 Aug;69(8):2340-2353. doi: 10.1099/ijsem.0.003474. Epub 2019 Jun 4.
Thirty Gram-stain-positive bacterial strains were isolated from Chinese traditional pickle. The strains were characterized using a polyphasic taxonomic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, fatty acid methyl ester analysis, determination of DNA G+C content, determination of average nucleotide identity (ANI), in silico DNA-DNA hybridization (isDDH), determination of average amino acid identity (AAI) and an analysis of phenotypic features. The data demonstrated that the 30 strains represented 11 novel species belonging to the genus Lactobacillus, strains 159-4, 47-3, 257-1, 187-3, 220-4, 151-2B, 137-3, 244-4, 218-10, 218-6 and 112-3 were designated as the type strains. Strains 159-4 and 47-3 were related to the type strains of Lactobacillus porcinae, Lactobacillus manihotivorans, Lactobacillus nasuensis, Lactobacillus camelliae, Lactobacillus pantheris, Lactobacillus thailandensis, Lactobacillus sharpeae and Lactobacillus songhuajiangensis, having 92.0-98.9 % 16S rRNA gene sequence similarities, 58.1-84.7 % pheS gene sequence similarities and 61.4-90.3 % rpoA gene sequence similarities. Strains 257-1 and 187-3 were related to the type strains of Lactobacillus coryniformis, Lactobacillus iwatensis, Lactobacillus backii, Lactobacillus rennini and Lactobacillus bifermentans, having 93.5-99.3 % 16S rRNA gene sequence similarities, 67.7-81.8 % pheS gene sequence similarities and 77.0-96.2 % rpoA gene sequence similarities. Strains 220-4, 151-2B, 137-3, 244-4, 218-10, 218-6 and 112-3 were closely related to the type strains of Lactobacillus paucivorans, Lactobacillus brevis, Lactobacillus hammesii, Lactobacillus senmaizukei, Lactobacillus parabrevis, Lactobacillus yonginensis, Lactobacillus koreensis and Lactobacillus cerevisiae, having 95.6-99.9 % 16S rRNA gene sequence similarities, less than 93.9 % pheS gene sequence similarities and 87.0-99.7 % rpoA gene sequence similarities. ANI, isDDH and AAI values between strains 159-4, 47-3, 257-1, 187-3, 220-4, 151-2B, 137-3, 244-4, 218-10, 218-6, 112-3 and type strains of phylogenetically related species were less than 92.7, 48.4 and 96.6 %, respectively, confirming that they represent 11 novel species within the genus Lactobacillus. Based upon the data of polyphasic characterization obtained in the present study, eleven novel species, Lactobacillusjixianensis sp. nov., Lactobacillusbaoqingensis sp. nov., Lactobacillusjiayinensis sp. nov., Lactobacilluszhaoyuanensis sp. nov., Lactobacilluslindianensis sp. nov., Lactobacillushuananensis sp. nov., Lactobacillustangyuanensis sp. nov., Lactobacillusfuyuanensis sp. nov., Lactobacillustongjiangensis sp. nov., Lactobacillusfujinensis sp. nov. and Lactobacillusmulengensis sp. nov., are proposed and the type strains are 159-4 (=NCIMB 15175=CCM 8911), 47-3 (=NCIMB 15165=CCM 8903=LMG 31064), 257-1 (=NCIMB 15166=CCM 8904=LMG 31065), 187-3 (=NCIMB 15172 =CCM 8910), 220-4 (=NCIMB 15163 =CCM 8902=KCTC 21136), 151-2B (=NCIMB 15164=CCM 8913=KCTC 21129=LMG 31063), 137-3 (=NCIMB 15170=CCM 8907=KCTC 21125=LMG 31053), 244-4 (=NCIMB 15168=CCM 8906=KCTC 21137=LMG 31052), 218-10 (=NCIMB 15167=CCM 8905=KCTC 21135 =LMG 31055), 218-6 (=NCIMB 15171=CCM 8908=KCTC 21134 =LMG 31067) and 112-3 (=NCIMB 15174=CCM 8909=KCTC 21123=LMG 31049), respectively.
三十株革兰氏阳性细菌从中国传统泡菜中分离得到。通过多相分类学方法，包括 16S rRNA 基因序列分析、pheS 基因序列分析、rpoA 基因序列分析、脂肪酸甲酯分析、DNA G+C 含量测定、平均核苷酸同一性 (ANI) 测定、DNA-DNA 杂交测定、平均氨基酸同一性 (AAI) 分析和表型特征分析，对这些菌株进行了鉴定。结果表明，这 30 株细菌代表了 11 种新的乳杆菌属物种，其中菌株 159-4、47-3、257-1、187-3、220-4、151-2B、137-3、244-4、218-10、218-6 和 112-3 被指定为模式菌株。菌株 159-4 和 47-3 与乳杆菌猪肠球菌、乳杆菌曼氏乳杆菌、乳杆菌那苏氏菌、乳杆菌山茶属、乳杆菌黑豹属、乳杆菌泰国亚种、乳杆菌 sharpeae 和乳杆菌松花江亚种的模式株有 92.0-98.9%的 16S rRNA 基因序列相似性、58.1-84.7%的 pheS 基因序列相似性和 61.4-90.3%的 rpoA 基因序列相似性。菌株 257-1 和 187-3 与乳杆菌 coryniformis、乳杆菌 iwatensis、乳杆菌 backii、乳杆菌 rennini 和乳杆菌 bifementans 的模式株有 93.5-99.3%的 16S rRNA 基因序列相似性、67.7-81.8%的 pheS 基因序列相似性和 77.0-96.2%的 rpoA 基因序列相似性。菌株 220-4、151-2B、137-3、244-4、218-10、218-6 和 112-3 与乳杆菌 paucivorans、乳杆菌 brevis、乳杆菌 hammesii、乳杆菌 senmaizukei、乳杆菌 parabrevis、乳杆菌 yonginensis、乳杆菌 koreensis 和乳杆菌 cerevisiae 的模式株密切相关，有 95.6-99.9%的 16S rRNA 基因序列相似性、不到 93.9%的 pheS 基因序列相似性和 87.0-99.7%的 rpoA 基因序列相似性。菌株 159-4、47-3、257-1、187-3、220-4、151-2B、137-3、244-4、218-10、218-6 和 112-3 与系统发育上相关物种的模式株之间的 ANI、isDDH 和 AAI 值分别小于 92.7%、48.4%和 96.6%，证实它们代表了乳杆菌属中的 11 个新种。基于本研究多相特征鉴定数据，提出了 11 个新种，即吉县乳杆菌 sp. nov.、保庆乳杆菌 sp. nov.、嘉荫乳杆菌 sp. nov.、赵源乳杆菌 sp. nov.、岭南乳杆菌 sp. nov.、环南海乳杆菌 sp. nov.、桃源乳杆菌 sp. nov.、富源乳杆菌 sp. nov.、通江乳杆菌 sp. nov.、傅晋乳杆菌 sp. nov.和牧棱乳杆菌 sp. nov.，其模式株分别为 159-4（=NCIMB 15175=CCM 8911）、47-3（=NCIMB 15165=CCM 8903=LMG 31064）、257-1（=NCIMB 15166=CCM 8904=LMG 31065）、187-3（=NCIMB 15172=CCM 8910）、220-4（=NCIMB 15163=CCM 8902=KCTC 21136）、151-2B（=NCIMB 15164=CCM 8913=KCTC 21129=LMG 31063）、137-3（=NCIMB 15170=CCM 8907=KCTC 21125=LMG 31053）、244-4（=NCIMB 15168=CCM 8906=KCTC 21137=LMG 31052）、218-10（=NCIMB 15167=CCM 8905=KCTC 21135=LMG 31055）、218-6（=NCIMB 15171=CCM 8908=KCTC 21134=LMG 31067）和 112-3（=NCIMB 15174=CCM 8909=KCTC 21123=LMG 31049）。
