Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
National Collection of Agricultural and Industrial Microorganisms, Faculty of Food Science, Corvinus University of Budapest, Somlói út 14-16. H-1118 Budapest, Hungary.
Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):3115-3123. doi: 10.1099/ijs.0.052589-0. Epub 2013 Jun 7.
Nine methanol-assimilating yeast strains isolated from olive oil sediments in Slovenia, extra virgin olive oil from Italy and rotten wood collected in Hungary were found to form three genetically separated groups, distinct from the currently recognized yeast species. Sequence analysis from genes of the small subunit (SSU) rRNA, internal transcribed spacer region/5.8S rRNA, large subunit (LSU) rRNA D1/D2 domains and translational elongation factor-1α (EF-1α) revealed that the three closely related groups represent three different undescribed yeast species. Sequence analysis of the LSU rRNA gene D1/D2 domains placed the novel species in the Ogataea clade. The three novel species are designated as Ogataea kolombanensis sp. nov. (type strain: ZIM 2322(T) = CBS 12778(T) = NRRL Y-63657(T)), Ogataea histrianica sp. nov. (type strain: ZIM 2463(T) = CBS 12779(T) = NRRL Y-63658(T)) and Ogataea deakii sp. nov. (type strain: NCAIM Y.01896(T) = CBS 12735(T) = NRRL Y-63656(T)).
从斯洛文尼亚橄榄油沉淀物、意大利特级初榨橄榄油和匈牙利腐烂木材中分离到的 9 株甲醇同化酵母菌株，形成了三个在遗传上分离的群体，与目前公认的酵母种不同。小亚基（SSU）rRNA、内部转录间隔区/5.8S rRNA、大亚基（LSU）rRNA D1/D2 区和翻译延伸因子-1α（EF-1α）的基因序列分析表明，这三个密切相关的群体代表了三个不同的未描述酵母种。LSU rRNA 基因 D1/D2 区的序列分析将新种归入 Ogataea 分支。这三个新种被命名为 Ogataea kolombanensis sp. nov.（模式株：ZIM 2322(T) = CBS 12778(T) = NRRL Y-63657(T)）、Ogataea histrianica sp. nov.（模式株：ZIM 2463(T) = CBS 12779(T) = NRRL Y-63658(T)）和 Ogataea deakii sp. nov.（模式株：NCAIM Y.01896(T) = CBS 12735(T) = NRRL Y-63656(T)）。
