Čadež Neža, Dlauchy Dénes, Tome Miha, Péter Gábor
Food Science and Technology Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
National Collection of Agricultural and Industrial Microorganisms, Faculty of Food Science, Szent István University, Somlói út 14-16, H-1118 Budapest, Hungary.
Microorganisms. 2021 Feb 2;9(2):301. doi: 10.3390/microorganisms9020301.
Taphrinomycotina is the smallest subphylum of the phylum Ascomycota. It is an assemblage of distantly related early diverging lineages of the phylum, comprising organisms with divergent morphology and ecology; however, phylogenomic analyses support its monophyly. In this study, we report the isolation of a yeast strain, which could not be assigned to any of the currently recognised five classes of Taphrinomycotina. The strain of the novel budding species was recovered from extra virgin olive oil and characterised phenotypically by standard methods. The ultrastructure of the cell wall was investigated by transmission electron microscopy. Comparisons of barcoding DNA sequences indicated that the investigated strain is not closely related to any known organism. Tentative phylogenetic placement was achieved by maximum-likelihood analysis of the D1/D2 domain of the nuclear LSU rRNA gene. The genome of the investigated strain was sequenced, assembled, and annotated. Phylogenomic analyses placed it next to the fission species. To accommodate the novel species, , a novel genus , a novel family Novakomycetaceae, a novel order Novakomycetales, and a novel class Novakomycetes is proposed as well. Functional analysis of genes missing in in comparison to revealed that they are biased towards biosynthesis of complex organic molecules, regulation of mRNA, and the electron transport chain. Correlating the genome content and physiology among species of Taphrinomycotina revealed some discordance between pheno- and genotype. produced ascospores in axenic culture preceded by conjugation between two cells. We confirmed that is a primary homothallic species lacking genes for different mating types.
座囊菌亚门是子囊菌门中最小的亚门。它是该门中亲缘关系较远、分化较早的谱系的集合，包含形态和生态各异的生物；然而，系统基因组分析支持其单系性。在本研究中，我们报告了一株酵母菌株的分离，该菌株无法归属于目前认可的座囊菌亚门的五个类别中的任何一类。从特级初榨橄榄油中分离出这种新型出芽物种的菌株，并通过标准方法对其进行表型特征分析。通过透射电子显微镜研究了细胞壁的超微结构。条形码DNA序列比较表明，所研究的菌株与任何已知生物的亲缘关系都不密切。通过对核 LSU rRNA 基因的 D1/D2 结构域进行最大似然分析，初步确定了其系统发育位置。对所研究菌株的基因组进行了测序、组装和注释。系统基因组分析将其置于裂殖酵母物种旁边。为了容纳这个新物种，还提出了一个新属、一个新科诺瓦科菌科、一个新目诺瓦菌目和一个新纲诺瓦菌纲。与其他物种相比，对该新物种中缺失基因的功能分析表明，它们偏向于复杂有机分子的生物合成、mRNA 的调控以及电子传递链。比较座囊菌亚门各物种的基因组含量和生理特征发现，表型和基因型之间存在一些不一致。该新物种在无菌培养中产生子囊孢子，之前两个细胞之间会发生接合。我们证实该新物种是一种初级同宗配合物种，缺乏不同交配型的基因。
