Department of Plant Molecular Biology and Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641003, India; Food Toxicology Division, Indian Institute of Toxicology Research (IITR), Mahatma Gandhi Marg, Lucknow, 226001, India.
J Sci Food Agric. 2014 Jan 30;94(2):308-15. doi: 10.1002/jsfa.6259. Epub 2013 Jul 17.
Several species of the genus Cassia are known for their antioxidant, antimicrobial and antidiabetic activities, but some of the lesser-known Cassia species, e.g. C. renigera, C. biflora and C. laevigata have not been studied for their biological activities.
Methanol extract of C. laevigata was fractionated by preparative thin-layer chromatography. The resulting six different fractions were tested against Fusarium oxysporum and Aspergillus niger for their antifungal activity. Due to higher antifungal activity of fraction 1 of C. laevigata, this was further analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC), resulting in distinct separation of one compound at a retention time of 7.2 min with an absorbance of 252 nm. Further, this compound was analyzed by gas chromatography-mass spectrometry (GC-MS) for its putative structural identification. Mass spectra of this compound resembled the spectra of anthraquinone 1-carboxylic acid by NIST library search. The genomic-level expression of chalcone synthase, a key enzyme involved in the biosynthesis of polyketides, was increased in C. laevigata when compared to other Cassia species.
This study provides an insight into the higher antifungal activity of C. laevigata, including the identification of anthraquinone 1-carboxylic acid, which may be responsible for the antifungal activity.
多种决明属植物因其抗氧化、抗菌和抗糖尿病活性而闻名，但一些不太知名的决明属植物，如 C. renigera、C. biflora 和 C. laevigata，其生物活性尚未得到研究。
C. laevigata 的甲醇提取物通过制备性薄层色谱进行了分离。所得的六种不同馏分被测试对 F. oxysporum 和 A. niger 的抗真菌活性。由于 C. laevigata 馏分 1 的抗真菌活性较高，因此进一步通过反相高效液相色谱（RP-HPLC）进行分析，在保留时间为 7.2 分钟时得到一个化合物的明显分离，其吸光度为 252nm。此外，通过气相色谱-质谱（GC-MS）对该化合物进行了可能的结构鉴定。该化合物的质谱与 NIST 库搜索的蒽醌 1-羧酸的质谱相似。与其他决明属植物相比，C. laevigata 中参与聚酮类生物合成的查尔酮合酶的基因组水平表达增加。
本研究深入了解了 C. laevigata 较高的抗真菌活性，包括鉴定出蒽醌 1-羧酸，这可能是其抗真菌活性的原因。
