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生物测定指导下从决明子种子提取物中分离具有抗酵母活性的活性化合物。

Bioassay-directed isolation of active compounds with antiyeast activity from a Cassia fistula seed extract.

机构信息

Biological Program, School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia.

出版信息

Molecules. 2011 Sep 5;16(9):7583-92. doi: 10.3390/molecules16097583.

DOI:10.3390/molecules16097583
PMID:21894090
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6264236/
Abstract

BACKGROUND AND OBJECTIVE

Cassia fistula L belongs to the family Leguminosae, and it is one of the most popular herbal products in tropical countries. C. fistula seeds have been used as a herbal medicine and have pharmacological activity which includes anti-bacterial, anti-fungal, and antioxidant properties. The goal of this study was to identify compounds from C. fistula seeds which are responsible for anti-Candida albicans activity using bioassay-directed isolation.

RESULTS

The preliminary phytochemical screening of the plant seed revealed the presence of anthraquinones, flavonoids, saponins, tannins and terpenoids. The isolation of active compounds was carried out in four steps: multiple extractions, fractionation using column chromatography and purification using preparative thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). The structure of separated compounds was determined on the basis of mass spectrometry data. One compound was identified is roseanone.

CONCLUSIONS

The MS analysis on the active fraction from seed extract of C. fistula confirmed the presence of roseanone with antiyeast activity.

摘要

背景与目的

决明子属于豆科植物，是热带国家最受欢迎的草药产品之一。决明子种子被用作草药，具有抗菌、抗真菌和抗氧化等药理活性。本研究的目的是使用生物活性导向分离法，从决明子种子中鉴定出具有抗白色念珠菌活性的化合物。

结果

对植物种子的初步植物化学筛选表明存在蒽醌类、黄酮类、皂苷类、鞣质和萜类化合物。活性化合物的分离采用了四步进行：多次提取、柱层析分离、制备性薄层层析（TLC）和液相色谱/质谱（LC/MS）纯化。根据质谱数据确定分离化合物的结构。分离出的一种化合物被鉴定为蔷薇酮。

结论

从决明子种子提取物的活性部分进行的 MS 分析证实了具有抗酵母活性的蔷薇酮的存在。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f3/6264236/6c05939c8cb7/molecules-16-07583-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f3/6264236/2822f79d3285/molecules-16-07583-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f3/6264236/d4a2cbf3d902/molecules-16-07583-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f3/6264236/5cd9ef4808c8/molecules-16-07583-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f3/6264236/6c05939c8cb7/molecules-16-07583-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f3/6264236/2822f79d3285/molecules-16-07583-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f3/6264236/d4a2cbf3d902/molecules-16-07583-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f3/6264236/5cd9ef4808c8/molecules-16-07583-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f3/6264236/6c05939c8cb7/molecules-16-07583-g004.jpg

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