Shen J, Zhang X J, Cong Z, Wang C C
Department of Physiology, Beijing Medical University.
Sheng Li Xue Bao. 1990 Apr;42(2):198-202.
We observed previously that indomethacin induced hepatic protection against CCl4 and galactosamine in rats. In this work we further investigated this phenomenon on primary cultured rat hepatocytes. The results showed that cultured hepatocytes of rats pretreated with indomethacin in vivo still retained the ability resisting CCl4-induced cytotoxicity, as indicated by lower leakage of enzymes. However, cultured hepatocytes of normal rats treated with indomethacin in vitro had no obvious resistance to CCl4-induced cytotoxicity. The results suggest that indomethacin pretreatment in vivo enables hepatocytes to resist injury, which probably also needs participation of other factors.
我们之前观察到，吲哚美辛可诱导大鼠肝脏对四氯化碳和半乳糖胺产生保护作用。在本研究中，我们进一步在原代培养的大鼠肝细胞上研究了这一现象。结果显示，体内经吲哚美辛预处理的大鼠培养肝细胞仍保留抵抗四氯化碳诱导的细胞毒性的能力，这表现为酶泄漏较少。然而，体外经吲哚美辛处理的正常大鼠培养肝细胞对四氯化碳诱导的细胞毒性没有明显的抵抗作用。结果表明，体内吲哚美辛预处理使肝细胞能够抵抗损伤，这可能还需要其他因素的参与。
