Keighron Jacqueline D, Keating Christine D
Pennsylvania State University, University Park, PA, USA.
Methods Mol Biol. 2013;1026:163-74. doi: 10.1007/978-1-62703-468-5_13.
Enzyme-gold nanoparticle bioconjugates have a wide variety of uses ranging from nanoreactors to sensors and model systems. While easy to make, these bioconjugates are often not well characterized. This protocol outlines preparation of enzyme-nanoparticle bioconjugates and two complementary methods for quantifying enzyme:nanoparticle stoichiometry from which the specific activity of the adsorbed enzymes can be determined. Characterizations such as these can aid researchers in improving the design and application of future bioconjugate systems.
酶-金纳米颗粒生物共轭物有多种用途，从纳米反应器到传感器以及模型系统。虽然这些生物共轭物易于制备，但它们的特性往往没有得到很好的表征。本方案概述了酶-纳米颗粒生物共轭物的制备方法以及两种互补的定量酶与纳米颗粒化学计量比的方法，由此可以确定吸附酶的比活性。这样的表征有助于研究人员改进未来生物共轭物系统的设计和应用。
