Hajdukiewicz Karolina, Stachurska Anna, Proczka Robert, Małecki Maciej
Department of Molecular Biology, Medical University of Warsaw, Warsaw, Poland.
Med Wieku Rozwoj. 2013 Jan-Mar;17(1):31-6.
In this study attention was focused on gene preparations that stimulate angiogenesis in the skin. Angiogenesis was stimulated by gene preparations encoding angiogenic factors introduced into the skin by injection, and applied as ointments.
The appropriate angiogenic formulations containing angiogenic nonviral vectors (pVEGF, pFGF, pSDF, pVIF), or viral vectors (rAAV/VEGF, rAAV/SDF) were prepared for the test. Cholesterol ointment was used as the vehicle for viral and non-viral vectors. The new vessels in the mouse skin were counted according to the criteria suggested by Sidky and Auerbach.
Studies indicate that all non-viral (pVEGF, pFGF, pSDF, pVIF) and viral (rAAV/VEGF, rAAV/SDF) vectors strongly stimulate new vessel formation when administered into mouse skin as injections. The impact of angiogenic gene ointments for skin angiogenesis was about 3-4 times weaker than that observed for injection preparations.
Angiogenic injection gene preparations strongly stimulate skin neovascularization. The clinical usefulness of gene ointments should stimulate further laboratory studies in the field of experimental skin gene therapy.
本研究聚焦于刺激皮肤血管生成的基因制剂。通过注射将编码血管生成因子的基因制剂引入皮肤，并制成软膏应用，以此刺激血管生成。
制备含血管生成非病毒载体（pVEGF、pFGF、pSDF、pVIF）或病毒载体（rAAV/VEGF、rAAV/SDF）的合适血管生成制剂用于试验。胆固醇软膏用作病毒和非病毒载体的载体。根据Sidky和Auerbach建议的标准对小鼠皮肤中的新血管进行计数。
研究表明，所有非病毒载体（pVEGF、pFGF、pSDF、pVIF）和病毒载体（rAAV/VEGF、rAAV/SDF）经注射施用于小鼠皮肤时，均强烈刺激新血管形成。血管生成基因软膏对皮肤血管生成的影响比注射制剂弱约3 - 4倍。
血管生成注射基因制剂强烈刺激皮肤新血管形成。基因软膏的临床实用性应推动实验性皮肤基因治疗领域的进一步实验室研究。
