Department of Obstetrics and Gynecology, LMU, University of Munich, Munich, Germany.
Anticancer Res. 2013 Jun;33(6):2491-8.
Measurement of cell survival as performed by the differential staining cytotoxicity (DiSC) assay allows for a comprehensive prediction of individual chemotherapy response. This assay has not reached routine clinical application because of its several limitations. In order to improve the DiSC assay, we developed a new type of chemotherapy sensitivity and resistance assay (CSRA) by combining a novel evaluation methodology with automated measurement of cell survival based on flow cytometry, termed AutoDiSC.
Tumour cell lines were treated with cytostatic drugs and cultured in 96-well plates for four days at 37 °C. Subsequently, propidium iodide was added and 96-well plates were analyzed using flow cytometry in order to determine the number of living cells in each well. Each component was evaluated for reproducibility and impact on assay read-out.
The evaluation process allowed for specification of all relevant parameters that determine assay performance.
AutoDiSC is a new fully-automated CSRA which meets all criteria required for routine clinical use.
通过差异染色细胞毒性（DiSC）检测法进行细胞存活测量，可以全面预测个体的化疗反应。由于该检测法存在多种局限性，因此尚未达到常规临床应用的程度。为了改进 DiSC 检测法，我们通过将一种新的评估方法与基于流式细胞术的细胞存活自动测量相结合，开发了一种新型的化疗敏感性和耐药性检测法（CSRA），称之为 AutoDiSC。
将肿瘤细胞系用细胞抑制剂药物处理，并在 37°C 下于 96 孔板中培养四天。然后，加入碘化丙啶，并使用流式细胞术分析 96 孔板，以确定每个孔中存活细胞的数量。评估每个组件的重复性及其对检测结果的影响。
评估过程确定了决定检测性能的所有相关参数。
AutoDiSC 是一种全新的全自动 CSRA，满足了常规临床应用所需的所有标准。
