Department of Orthopedics, The Fifth Central Hospital of Tianjin, Tianjin, China.
Spinal Cord. 2013 Aug;51(8):610-5. doi: 10.1038/sc.2013.24. Epub 2013 Jun 11.
Spinal cord injury (SCI) is a devastating and common neurologic disorder that has profound influences on modern society from physical, psychosocial and socio-economic perspectives.
To analyze the dynamic changes in protein expression during SCI after ischemia-reperfusion.
We used two-dimensional difference gel electrophoresis combined with matrix-assisted laser desorption/ionization time-of-flight/time-of-flight MS to give a global analysis of protein dynamic change during SCI after ischemia-reperfusion. Dynamic changes in protein expression were investigated from 6 to 48 h in SCI after ischemia-reperfusion using a proteomics tool.
Twenty-one proteins were identified in total, including neuronal proteins, glycometabolism enzymes, stress-related proteins and cytoskeleton-related proteins. These were divided into upregulated and downregulated groups. Results identified 24 h as a key time point when all proteins were changed dramatically. In addition, changes in Fascin expression were discovered in SCI for the first time.
In conclusion, we observed dynamic proteome change correlated with SCI by ischemia-reperfusion, and provided a clue to this pathological mechanism by protein identification and analysis.
脊髓损伤（SCI）是一种破坏性的常见神经系统疾病，从生理、心理社会和社会经济的角度来看，它对现代社会有深远的影响。
分析 SCI 缺血再灌注后蛋白质表达的动态变化。
我们使用二维差异凝胶电泳结合基质辅助激光解吸/电离飞行时间/飞行时间 MS 对 SCI 缺血再灌注后蛋白质的动态变化进行了全面分析。使用蛋白质组学工具研究了 SCI 缺血再灌注后 6 至 48 小时蛋白质表达的动态变化。
共鉴定出 21 种蛋白质，包括神经元蛋白、糖代谢酶、应激相关蛋白和细胞骨架相关蛋白。这些蛋白质分为上调和下调两组。结果表明，24 小时是所有蛋白质发生剧烈变化的关键时间点。此外，还首次在 SCI 中发现 Fascin 表达的变化。
总之，我们通过缺血再灌注观察到与 SCI 相关的动态蛋白质组变化，并通过蛋白质鉴定和分析为该病理机制提供了线索。
