Chopinet Louise, Roduit Charles, Rols Marie-Pierre, Dague Etienne
CNRS, LAAS, 7 avenue du colonel Roche, F-31400 Toulouse, France.
Biochim Biophys Acta. 2013 Sep;1828(9):2223-9. doi: 10.1016/j.bbamem.2013.05.035. Epub 2013 Jun 10.
Electropermeabilization is a physical method that uses electric field pulses to deliver molecules into cells and tissues. Despite its increasing interest in clinics, little is known about plasma membrane destabilization process occurring during electropermeabilization. In this work, we took advantage of atomic force microscopy to directly visualize the consequences of electropermeabilization in terms of membrane reorganization and to locally measure the membrane elasticity. We visualized transient rippling of membrane surface and measured a decrease in membrane elasticity by 40%. Our results obtained both on fixed and living CHO cells give evidence of an inner effect affecting the entire cell surface that may be related to cytoskeleton destabilization. Thus, AFM appears as a useful tool to investigate basic process of electroporation on living cells in absence of any staining or cell preparation.
电穿孔是一种利用电场脉冲将分子导入细胞和组织的物理方法。尽管临床上对其兴趣日益增加，但对于电穿孔过程中发生的质膜去稳定化过程却知之甚少。在这项工作中，我们利用原子力显微镜直接观察电穿孔在膜重组方面的后果，并局部测量膜弹性。我们观察到膜表面的瞬时波动，并测量到膜弹性下降了40%。我们在固定和活的CHO细胞上获得的结果证明了一种影响整个细胞表面的内在效应，这可能与细胞骨架去稳定化有关。因此，原子力显微镜似乎是一种有用的工具，可用于在不进行任何染色或细胞制备的情况下研究活细胞上电穿孔的基本过程。
