纳秒级脉冲电场对细胞弹性的影响。
Effects of nanosecond pulse electric fields on cellular elasticity.
作者信息
Dutta Diganta, Asmar Anthony, Stacey Michael
机构信息
Institute of Micro and Nanotechnology, Mechanical and Aerospace Engineering Department, Old Dominion University, Norfolk, VA, USA.
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA.
出版信息
Micron. 2015 May;72:15-20. doi: 10.1016/j.micron.2015.01.004. Epub 2015 Feb 13.
We investigated the effects of a single 60 nanosecond pulsed electric field (nsPEF) of low (15 kV/cm) and high (60 kV/cm) field strengths on cellular morphology and membrane elasticity in Jurkat cells using fluorescent microscopy and atomic force microscopy (AFM). We performed force displacement measurements on cells using AFM and calculated the Young's modulus for membrane elasticity. Differential effects were observed depending upon pulsing conditions. We found that a single nsPEF of low field strength did not induce any apparent cytoskeletal breakdown and had minor morphological changes. Interestingly, force measurements and calculation of Young's modulus showed a significant decrease in membrane elasticity. A single nsPEF of high field strength induced stark morphological changes due to disruption of the actin cytoskeleton and a marked decrease in elasticity likely caused by irreversible membrane damage. We suggest that the cellular morphology is mainly dependent on stabilization by the actin cytoskeleton, while the elasticity changes are partially dependent on the cytoskeletal integrity.
我们使用荧光显微镜和原子力显微镜（AFM）研究了低场强（15 kV/cm）和高场强（60 kV/cm）的单个60纳秒脉冲电场（nsPEF）对Jurkat细胞的细胞形态和膜弹性的影响。我们使用AFM对细胞进行了力位移测量，并计算了膜弹性的杨氏模量。根据脉冲条件观察到了不同的效应。我们发现，低场强的单个nsPEF不会引起任何明显的细胞骨架破坏，且形态变化较小。有趣的是，力测量和杨氏模量计算显示膜弹性显著降低。高场强的单个nsPEF由于肌动蛋白细胞骨架的破坏而引起明显的形态变化，弹性显著降低可能是由不可逆的膜损伤所致。我们认为，细胞形态主要依赖于肌动蛋白细胞骨架的稳定作用，而弹性变化部分依赖于细胞骨架的完整性。
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