Edwin Shiaw C S, Shiran M S, Cheah Y K, Tan G C, Sabariah A R
University Putra Malaysia, Faculty of Medicine and Health Sciences, Department of Biomedical Sciences, 43400 UPM Serdang, Selangor, Malaysia.
Med J Malaysia. 2010 Jun;65(2):133-7.
This study was done to evaluate various DNA and RNA extractions from archival FFPE tissues. A total of 30 FFPE blocks from the years of 2004 to 2006 were assessed with each modified and adapted method. Extraction protocols evaluated include the modified enzymatic extraction method (Method A), Chelex-100 extraction method (Method B), heat-induced retrieval in alkaline solution extraction method (Methods C and D) and one commercial FFPE DNA Extraction kit (Qiagen, Crawley, UK). For RNA extraction, 2 extraction protocols were evaluated including the enzymatic extraction method (Method 1), and Chelex-100 RNA extraction method (Method 2). Results show that the modified enzymatic extraction method (Method A) is an efficient DNA extraction protocol, while for RNA extraction, the enzymatic method (Method 1) and the Chelex-100 RNA extraction method (Method 2) are equally efficient RNA extraction protocols.
本研究旨在评估从存档福尔马林固定石蜡包埋（FFPE）组织中提取各种DNA和RNA的方法。采用每种改良和适配方法对2004年至2006年的30个FFPE组织块进行了评估。评估的提取方案包括改良酶提取法（方法A）、Chelex-100提取法（方法B）、碱性溶液热诱导复苏提取法（方法C和D）以及一种商业FFPE DNA提取试剂盒（Qiagen，英国克劳利）。对于RNA提取，评估了2种提取方案，包括酶提取法（方法1）和Chelex-100 RNA提取法（方法2）。结果表明，改良酶提取法（方法A）是一种高效的DNA提取方案，而对于RNA提取，酶法（方法1）和Chelex-100 RNA提取法（方法2）是同样高效的RNA提取方案。
