Limnological Observatory of the Pyrenees (LOOP) - Biogeodynamics & Biodiversity Group, Centro de Estudios Avanzados de Blanes, CEAB-CSIC, Accés Cala Sant Francesc, 14, 17300 Blanes, Girona, Spain.
Environ Microbiol Rep. 2012 Feb;4(1):50-6. doi: 10.1111/j.1758-2229.2011.00278.x. Epub 2011 Aug 19.
Bacterial community composition was analysed in the slush layers of snow-covered Lake Redon (2240 m altitude, Limnological Observatory of the Pyrenees, LOOP, NE Spain) in winter and spring and compared with bacteria from the lake water column, using 16S rRNA gene clone libraries and CARD-FISH counts. The set of biological data was related to changes in bacterial production and to other relevant environmental variables measured in situ. In winter, up to 70% of the 16S rRNA sequences found in the slush were closely related to planktonic bacteria from the water column beneath the ice. Conversely, during spring ablation, 50% of the sequences had > 97% identity with bacteria from the cryosphere (i.e. globally distributed glaciers, snow and ice) and may have originated from remote aerosol deposition. The transition winter to spring was characterized by consistent community changes switching from assemblages dominated by Betaproteobacteria, Verrucomicrobia and Bacteroidetes during snowpack growth to communities essentially dominated by the Bacteroidetes of classes Cytophagia and Sphingobacteria. This strong bacterial composition switch was associated with consistent increases in bacterial abundance and production, and decreasing bacterial diversity.
在冬季和春季，对西班牙东北部比利牛斯山脉湖沼学观测站（LOOP）中海拔 2240 米被雪覆盖的雷东湖（Lake Redon）的浆层中的细菌群落组成进行了分析，并与湖水柱中的细菌进行了比较，使用了 16S rRNA 基因克隆文库和 CARD-FISH 计数。这组生物数据与细菌生产力的变化以及现场测量的其他相关环境变量有关。在冬季，在浆层中发现的多达 70%的 16S rRNA 序列与冰下水柱中的浮游细菌密切相关。相反，在春季融雪期间，有 50%的序列与来自冰冻圈（即全球分布的冰川、雪和冰）的细菌具有>97%的同一性，可能来自远程气溶胶沉积。从冬季到春季的转变以群落变化为特征，从雪包生长期间以β变形菌门、疣微菌门和拟杆菌门为主的组合转变为主要由浮霉菌门和鞘脂杆菌门的类群组成的组合。这种强烈的细菌组成转换与细菌丰度和生产力的持续增加以及细菌多样性的减少有关。
