PIF4 和 PIF5 转录因子将蓝光和生长素联系起来，调节拟南芥的向光性反应。
PIF4 and PIF5 transcription factors link blue light and auxin to regulate the phototropic response in Arabidopsis.
机构信息
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
出版信息
Plant Cell. 2013 Jun;25(6):2102-14. doi: 10.1105/tpc.113.112417. Epub 2013 Jun 11.
Abstract
Both blue light (BL) and auxin are essential for phototropism in Arabidopsis thaliana. However, the mechanisms by which light is molecularly linked to auxin during phototropism remain elusive. Here, we report that phytochrome interacting factoR4 (PIF4) and PIF5 act downstream of the BL sensor phototropin1 (PHOT1) to negatively modulate phototropism in Arabidopsis. We also reveal that PIF4 and PIF5 negatively regulate auxin signaling. Furthermore, we demonstrate that PIF4 directly activates the expression of the auxin/indole-3-acetic acid (IAA) genes IAA19 and IAA29 by binding to the G-box (CACGTG) motifs in their promoters. Our genetic assays demonstrate that IAA19 and IAA29, which physically interact with auxin response factor7 (ARF7), are sufficient for PIF4 to negatively regulate auxin signaling and phototropism. This study identifies a key step of phototropic signaling in Arabidopsis by showing that PIF4 and PIF5 link light and auxin.
摘要
蓝光（BL）和生长素对于拟南芥的向光性都是必不可少的。然而，光在向光性过程中如何与生长素分子结合的机制仍不清楚。在这里，我们报告说，光敏色素相互作用因子 4（PIF4）和 PIF5 作为 BL 传感器光受体 1（PHOT1）的下游因子，负调控拟南芥的向光性。我们还揭示了 PIF4 和 PIF5 负调控生长素信号。此外，我们证明 PIF4 通过结合其启动子中的 G 盒（CACGTG）基序，直接激活生长素/吲哚-3-乙酸（IAA）基因 IAA19 和 IAA29 的表达。我们的遗传分析表明，IAA19 和 IAA29 与生长素响应因子 7（ARF7）物理相互作用，足以使 PIF4 负调控生长素信号和向光性。本研究通过表明 PIF4 和 PIF5 连接光和生长素，确定了拟南芥向光性信号的一个关键步骤。
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