Wang Yixuan, Zhou Zhongyang, Liu Xiaomin
State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Plant Cell Rep. 2024 Dec 31;44(1):20. doi: 10.1007/s00299-024-03415-4.
Auxin stimulates chloroplast division by upregulating the expression of genes involved in chloroplast division and influencing the positioning of chloroplast division rings. Chloroplasts divide by binary fission, forming a ring complex at the division site. Auxin, particularly indole acetic acid (IAA), significantly influences various aspects of plant growth. However, the impact of auxin on chloroplast division remains unclear. In this study, different concentrations of exogenous IAA were applied to wild Arabidopsis thaliana. The results showed that the number and size differences of chloroplasts in the cells of Arabidopsis thaliana treated with high concentrations of IAA increased compared to untreated plants. Further investigation revealed that high concentrations of IAA affected the expression of chloroplast division genes and the formation of division rings. In chloroplast division mutants, the effect of IAA on promoting chloroplast division is impaired. Defects of IAA synthetic gene also lead to a reduced effect of IAA on chloroplast division. Our findings demonstrate that auxin influences chloroplast division by regulating the expressions of chloroplast division genes and affecting the localization of division rings. These results are significant for further exploring the relationship between auxin and chloroplast division.
生长素通过上调参与叶绿体分裂的基因表达并影响叶绿体分裂环的定位来刺激叶绿体分裂。叶绿体通过二分裂进行分裂，在分裂位点形成一个环状复合体。生长素，尤其是吲哚乙酸（IAA），对植物生长的各个方面都有显著影响。然而，生长素对叶绿体分裂的影响仍不清楚。在本研究中，将不同浓度的外源IAA施加到野生拟南芥上。结果表明，与未处理的植物相比，用高浓度IAA处理的拟南芥细胞中叶绿体的数量和大小差异增加。进一步研究发现，高浓度IAA影响叶绿体分裂基因的表达和分裂环的形成。在叶绿体分裂突变体中，IAA促进叶绿体分裂的作用受损。IAA合成基因的缺陷也导致IAA对叶绿体分裂的作用减弱。我们的研究结果表明，生长素通过调节叶绿体分裂基因的表达和影响分裂环的定位来影响叶绿体分裂。这些结果对于进一步探索生长素与叶绿体分裂之间的关系具有重要意义。
