Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Örebro, Sweden.
APMIS. 2014 Feb;122(2):155-60. doi: 10.1111/apm.12121. Epub 2013 Jun 12.
An outbreak of Clostridium difficile infection (CDI) at Höglandet Hospital Eksjö in southern Sweden in 2011 was mainly due to a multidrug-resistant PCR ribotype 046 (30% of all samples). Diagnostics used routinely was the Vidas CDAB assay, but to control the outbreak the rapid loop-mediated isothermal amplification (LAMP) assay Illumigene was introduced and both techniques were compared to Toxigenic culture (TC) prospectively. The LAMP assay had a superior sensitivity, that is, 98% compared to 79% for the Vidas CDAB assay. Most importantly, the mean turn-around-time from collecting sample to result was reduced from 59 h to 2 h enabling early isolation of patients and effective hygiene precautions. This may potentially decrease the morbidity and nosocomial transmissions of C. difficile.
2011 年，瑞典南部埃克斯约的 Höglandet 医院爆发艰难梭菌感染（CDI），主要是由于一种多药耐药的 PCR 核糖体 046 型（占所有样本的 30%）。常规使用的诊断方法是 Vidas CDAB 检测，但为了控制疫情，引入了快速环介导等温扩增（LAMP）检测 Illumigene，并前瞻性地将这两种技术与产毒培养（TC）进行了比较。LAMP 检测的灵敏度更高，为 98%，而 Vidas CDAB 检测的灵敏度为 79%。最重要的是，从采集样本到得出结果的平均周转时间从 59 小时缩短到 2 小时，从而能够更早地隔离患者并采取有效的卫生预防措施。这可能会降低艰难梭菌的发病率和医院内传播。
