CDK20 过表达预示结直肠癌患者预后不良。
CDC20 overexpression predicts a poor prognosis for patients with colorectal cancer.
机构信息
State Key Laboratory of Oncology in Southern China, Guangzhou 510060, China.
出版信息
J Transl Med. 2013 Jun 10;11:142. doi: 10.1186/1479-5876-11-142.
BACKGROUND
The cell division cycle 20 homolog (CDC20) is an essential cofactor of the anaphase-promoting complex (APC/C). CDC20 overexpression has been detected in many types of human cancers; however, its clinical role in colorectal cancer remains unknown.
METHODS
Western blotting and immunohistochemistry were used to compare CDC20 expression in adjacent non-cancerous, cancerous and liver metastatic tissues as well as in colon cancer cell lines and normal colon epithelial cell lines. Additionally, the correlation of CDC20 expression with patient clinical parameters and its diagnostic value were statistically analyzed.
RESULTS
CDC20 was overexpressed in colon cancer cell lines/primary cancer tissues compared with normal colon epithelial cell lines/adjacent noncancerous tissue samples. Interestingly, CDC20 expression was further increased in metastatic liver tissues. CDC20 protein expression was significantly correlated with clinical stage (P = 0.008), N classification (P = 0.020), M classification (P = 0.013) and pathologic differentiation (P = 0.008). Patients with higher CDC20 expression had a shorter overall survival than those with lower CDC20 expression. Univariate and multivariate analyses indicated that CDC20 expression was an independent prognostic factor (P < 0.001).
CONCLUSION
CDC20 may serve as a potential prognostic biomarker of human colorectal cancer.
背景
细胞分裂周期 20 同源物（CDC20）是后期促进复合物（APC/C）的必需辅助因子。在许多类型的人类癌症中都检测到 CDC20 过表达；然而，其在结直肠癌中的临床作用尚不清楚。
方法
使用 Western blot 和免疫组织化学比较相邻非癌性、癌性和肝转移组织以及结肠癌细胞系和正常结肠上皮细胞系中 CDC20 的表达。此外，还对 CDC20 表达与患者临床参数的相关性及其诊断价值进行了统计学分析。
结果
与正常结肠上皮细胞系/相邻非癌组织样本相比，CDC20 在结肠癌细胞系/原发性癌组织中过表达。有趣的是，CDC20 的表达在转移性肝组织中进一步增加。CDC20 蛋白表达与临床分期（P = 0.008）、N 分类（P = 0.020）、M 分类（P = 0.013）和病理分化（P = 0.008）显著相关。CDC20 表达较高的患者总生存期短于 CDC20 表达较低的患者。单因素和多因素分析表明，CDC20 表达是独立的预后因素（P < 0.001）。
结论
CDC20 可能是人类结直肠癌的潜在预后生物标志物。
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