VA Center of Excellence for Stress and Mental Health, San Diego, CA, United States; University of California San Diego, San Diego, CA, United States; VA San Diego Healthcare System, San Diego, CA, United States.
Psychoneuroendocrinology. 2013 Oct;38(10):2378-82. doi: 10.1016/j.psyneuen.2013.04.020. Epub 2013 Jun 10.
Neuropeptide Y (NPY) is abundant in mammals, where it contributes to diverse behavioral and physiological functions, centrally and peripherally, but little information is available in regard to NPY cerebrospinal fluid (CSF)/plasma concentration relationships and dynamics. Since plasma NPY levels are commonly used as proxy "biomarkers" for central NPY activity in stress and mental health research in humans this study aims to better characterize the CSF/plasma NPY relationships. Subjects were eleven healthy male volunteers, admitted to the clinical research center for placement of an indwelling CSF catheter, as well as venous catheter, for 24-h collection of CSF NPY (cNPY) and plasma NPY (pNPY) samples. As observed in prior studies, group mean (SE) cNPY concentrations [792.1 (7.80) pg/mL] were higher than pNPY concentrations [220.0 (3.63) pg/mL]. For the eleven normal volunteers who had sufficient common (hourly) pNPY and cNPY data points, analysis of pNPY/cNPY concentration ratios and lagged cross-correlation analysis was completed. Average pNPY/cNPY concentration ratios ranged from .20 to .40 across study subjects, with a mean of .29. pNPY/cNPY cross correlation analyses, computed at varying time lags, were non-significant. An attempt was made to analyze the circadian rhythmicity of NPY secretion, but circadian components were not detectable. Using 24-h data collection, we characterized CSF/plasma NPY relationships, including presentation of evidence of weak CSF and plasma correlations, an important consideration for study design of NPY in stress or mental health.
神经肽 Y（NPY）在哺乳动物中含量丰富，它在中枢和外周神经系统中参与多种行为和生理功能。然而，关于 NPY 脑脊液（CSF）/血浆浓度关系和动力学的信息却很少。由于在人类应激和心理健康研究中，血浆 NPY 水平通常被用作中枢 NPY 活性的替代“生物标志物”，因此本研究旨在更好地描述 CSF/血浆 NPY 关系。研究对象为 11 名健康男性志愿者，他们被收入临床研究中心，以放置一个留置 CSF 导管和静脉导管，用于 24 小时采集 CSF NPY（cNPY）和血浆 NPY（pNPY）样本。如先前研究中观察到的那样，组平均（SE）cNPY 浓度[792.1（7.80）pg/mL]高于 pNPY 浓度[220.0（3.63）pg/mL]。对于 11 名有足够共同（每小时）pNPY 和 cNPY 数据点的正常志愿者，完成了 pNPY/cNPY 浓度比和滞后交叉相关分析。研究对象的平均 pNPY/cNPY 浓度比范围在 0.20 到 0.40 之间，平均为 0.29。在不同的时间延迟下计算的 pNPY/cNPY 交叉相关分析没有显著意义。尝试分析 NPY 分泌的昼夜节律性，但无法检测到昼夜节律成分。使用 24 小时数据采集，我们描述了 CSF/血浆 NPY 关系，包括呈现 CSF 和血浆相关性较弱的证据，这是应激或心理健康中 NPY 研究设计的一个重要考虑因素。
