Nimesh Surendra, Mohottalage Susantha, Vincent Renaud, Kumarathasan Prem
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, ON K1A0K9, Canada.
Int J Mol Sci. 2013 May 28;14(6):11277-301. doi: 10.3390/ijms140611277.
Mass spectrometry imaging is employed for mapping proteins, lipids and metabolites in biological tissues in a morphological context. Although initially developed as a tool for biomarker discovery by imaging the distribution of protein/peptide in tissue sections, the high sensitivity and molecular specificity of this technique have enabled its application to biomolecules, other than proteins, even in cells, latent finger prints and whole organisms. Relatively simple, with no requirement for labelling, homogenization, extraction or reconstitution, the technique has found a variety of applications in molecular biology, pathology, pharmacology and toxicology. By discriminating the spatial distribution of biomolecules in serial sections of tissues, biomarkers of lesions and the biological responses to stressors or diseases can be better understood in the context of structure and function. In this review, we have discussed the advances in the different aspects of mass spectrometry imaging processes, application towards different disciplines and relevance to the field of toxicology.
质谱成像技术用于在形态学背景下对生物组织中的蛋白质、脂质和代谢物进行图谱绘制。尽管该技术最初是作为一种通过对组织切片中蛋白质/肽的分布进行成像来发现生物标志物的工具而开发的，但由于其高灵敏度和分子特异性，它已能够应用于除蛋白质之外的生物分子，甚至可用于细胞、潜在指纹和整个生物体。该技术相对简单，无需标记、匀浆、提取或重构，已在分子生物学、病理学、药理学和毒理学等领域得到了广泛应用。通过区分组织连续切片中生物分子的空间分布，可以在结构和功能的背景下更好地理解病变的生物标志物以及对应激源或疾病的生物学反应。在这篇综述中，我们讨论了质谱成像过程不同方面的进展、在不同学科中的应用以及与毒理学领域的相关性。
