Ye Hui, Greer Tyler, Li Lingjun
Department of Chemistry & School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA.
Bioanalysis. 2011 Feb;3(3):313-32. doi: 10.4155/bio.10.201.
Three dimensional mass spectral imaging (3D MSI) is an exciting field that grants the ability to study a broad mass range of molecular species ranging from small molecules to large proteins by creating lateral and vertical distribution maps of select compounds. Although the general premise behind 3D MSI is simple, factors such as choice of ionization method, sample handling, software considerations and many others must be taken into account for the successful design of a 3D MSI experiment. This review provides a brief overview of ionization methods, sample preparation, software types and technological advancements driving 3D MSI research of a wide range of low- to high-mass analytes. Future perspectives in this field are also provided to conclude that the outlook for 3D MSI is positive and promises ever-growing applications in the biomedical field with continuous developments of this powerful analytical tool.
三维质谱成像（3D MSI）是一个令人兴奋的领域，它能够通过创建选定化合物的横向和纵向分布图，研究从小分子到大型蛋白质等广泛质量范围内的分子种类。尽管3D MSI背后的一般原理很简单，但要成功设计一个3D MSI实验，必须考虑诸如电离方法的选择、样品处理、软件考量等诸多因素。本综述简要概述了电离方法、样品制备、软件类型以及推动对各种低质量至高质量分析物进行3D MSI研究的技术进展。还提供了该领域的未来展望，得出结论：随着这一强大分析工具的不断发展，3D MSI的前景乐观，有望在生物医学领域得到越来越广泛的应用。
