不同采收时间凤仙花茎叶乙醇提取物的抗氧化和抗菌活性研究（凤仙花科）。
Antioxidant and antimicrobial activities of ethanol extract from the stem and leaf of Impatiens balsamina L. (Balsaminaceae) at different harvest times.
机构信息
Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, Gyeongnam 660-758, Korea.
出版信息
Molecules. 2013 May 29;18(6):6356-65. doi: 10.3390/molecules18066356.
The aim of this study was to investigate the total phenolic content, total flavonoid contents, antioxidant activity and antimicrobial activity of ethanolic extract from stems (S) and leaves (L) of Impatiens balsamina L. (Balsaminaceae), which were harvested in Korea on March 10, 2011 (S1 and L1), May 14, 2011 (S2 and L2), and July 5, 2011 (S3 and L3), respectively. Our results revealed that the total phenolic (79.55-103.94 mg CE/g extract) and flavonoid (57.43-104.28 mg QE/g extract) contents of leaf extract were higher (p < 0.01) than those of stem extract. Leaf extracts (L1, L2, and L3) exhibited stronger (p < 0.01) free radical scavenging activity (66.06, 63.71, and 72.19%, respectively) than that of the positive control. In terms of antimicrobial activity, leaf extracts showed higher inhibitory effects against microorganisms than those of stem extracts (S1, S2, and S3). Among the leaf extracts at different harvest times, L3 showed the greatest antimicrobial activity against both Gram negative and Gram positive strains. From these results, the leaf extract from I. balsamina L. might be a valuable bioactive resource, and would seem to be applicable as a natural antioxidant in food preservation.
本研究旨在探讨于 2011 年 3 月 10 日（S1 和 L1）、5 月 14 日（S2 和 L2）和 7 月 5 日（S3 和 L3）分别采集于韩国的凤仙花（Impatiens balsamina L.）茎（S）和叶（L）的乙醇提取物的总酚含量、总黄酮含量、抗氧化活性和抗菌活性。我们的结果表明，叶提取物的总酚（79.55-103.94mgCE/g 提取物）和类黄酮（57.43-104.28mgQE/g 提取物）含量高于茎提取物（p<0.01）。叶提取物（L1、L2 和 L3）的自由基清除活性（分别为 66.06%、63.71%和 72.19%）明显强于阳性对照物（p<0.01）。在抗菌活性方面，叶提取物对微生物的抑制作用强于茎提取物（S1、S2 和 S3）。在不同收获时间的叶提取物中，L3 对革兰氏阴性和革兰氏阳性菌株均表现出最大的抗菌活性。从这些结果来看，凤仙花叶提取物可能是一种有价值的生物活性资源，并且似乎可以作为食品保鲜中的天然抗氧化剂。
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