Lucas J H, Wang G F, Gross G W
Department of Biological Sciences, University of North Texas, Denton 76203.
Brain Res. 1990 May 28;517(1-2):354-7. doi: 10.1016/0006-8993(90)91051-h.
Survival of cultured spinal neurons 2 h after dendrotomy increased significantly when the temperature was reduced to 17 degrees C or 7 degrees C. Survival of lesioned neurons maintained at 17 degrees C for 2 h and then at 37 degrees C for 22 h was still significantly higher than survival of neurons maintained at 37 degrees C for 24 h. Below 17 degrees C both lesioned and uninjured neurons swelled. Upon rewarming to 37 degrees C, many swollen neurons died. Ketamine prevented hypothermic swelling and neuronal death.
当温度降至17摄氏度或7摄氏度时，切断树突后2小时培养的脊髓神经元存活率显著提高。损伤神经元在17摄氏度下维持2小时，然后在37摄氏度下维持22小时，其存活率仍显著高于在37摄氏度下维持24小时的神经元存活率。在17摄氏度以下，损伤和未损伤的神经元都会肿胀。复温至37摄氏度时，许多肿胀的神经元死亡。氯胺酮可防止低温性肿胀和神经元死亡。
